Carcinogenesis, Vol 19, 1157-1159, Copyright © 1998 by Oxford University Press
SW Lee, CL Reimer, DB Campbell, P Cheresh, RB Duda and O Kocher
H-cadherin is a newly characterized cadherin molecule whose expression is
decreased in a variety of human carcinoma cells, suggesting that it may
play a role in maintaining normal cellular phenotype. To investigate how
re-expression of H-cadherin could influence the malignant phenotype of
human breast carcinoma cells in vivo, we transfected both control and
H-cadherin expression vectors into human breast cancer cells (MDAMB435),
which do not express H-cadherin constitutively. We found that invasiveness
of these cells could be prevented by transfection with H-cadherin. We also
compared the ability of control- and H-cadherin-transfected cells to induce
subcutaneous tumors after injection into mammary fat pads of nude mice. Our
results show that H-cadherin transfection produced a marked inhibition of
tumor growth and modified the morphology of tumor cells: tumors from mice
injected with control cells were significantly larger and contained larger
cells having a higher degree of pleomorphism than those of tumors generated
from carcinoma cells expressing H-cadherin. Altogether, these results
indicate that H-cadherin expression antagonizes tumor growth in nude mice,
presumably by enhancing cell- cell association in a tissue environment.
These findings strongly suggest that H-cadherin could provide a possible
target for corrective gene therapy against breast cancer.
ARTICLES
H-cadherin expression inhibits in vitro invasiveness and tumor formation in vivo
Department of Medicine, Beth Israel Deaconess Medical Center, Harvard Medical School and Harvard Institutes of Medicine, Boston, MA 02115, USA. slee2@bidmc.harvard.edu
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Yamamoto, M. Toyota, H. Suzuki, Y. Kondo, T. Sanomura, Y. Murayama, M. Ohe-Toyota, R. Maruyama, M. Nojima, M. Ashida, et al. LINE-1 Hypomethylation Is Associated with Increased CpG Island Methylation in Helicobacter pylori-Related Enlarged-Fold Gastritis Cancer Epidemiol. Biomarkers Prev., October 1, 2008; 17(10): 2555 - 2564. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Dames, E. Bang, D. Haussinger, T. Ahrens, J. Engel, and S. Grzesiek Insights into the Low Adhesive Capacity of Human T-cadherin from the NMR Structure of Its N-terminal Extracellular Domain J. Biol. Chem., August 22, 2008; 283(34): 23485 - 23495. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Miotto, S. Sabbioni, A. Veronese, G. A. Calin, S. Gullini, A. Liboni, L. Gramantieri, L. Bolondi, E. Ferrazzi, R. Gafa, et al. Frequent Aberrant Methylation of the CDH4 Gene Promoter in Human Colorectal and Gastric Cancer Cancer Res., November 15, 2004; 64(22): 8156 - 8159. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Roman-Gomez, J. A. Castillejo, A. Jimenez, F. Cervantes, C. Boque, L. Hermosin, A. Leon, A. Granena, D. Colomer, A. Heiniger, et al. Cadherin-13, a Mediator of Calcium-Dependent Cell-Cell Adhesion, Is Silenced by Methylation in Chronic Myeloid Leukemia and Correlates With Pretreatment Risk Profile and Cytogenetic Response to Interferon Alfa J. Clin. Oncol., April 15, 2003; 21(8): 1472 - 1479. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Qin, Z. Zeng, J. Zheng, P. K. Shah, S. M. Schwartz, L. D. Adams, and B. G. Sharifi Suppression Subtractive Hybridization Identifies Distinctive Expression Markers for Coronary and Internal Mammary Arteries Arterioscler Thromb Vasc Biol, March 1, 2003; 23(3): 425 - 433. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Z.-y. Huang, Y. Wu, N. Hedrick, and D. H. Gutmann T-Cadherin-Mediated Cell Growth Regulation Involves G2 Phase Arrest and Requires p21CIP1/WAF1 Expression Mol. Cell. Biol., January 15, 2003; 23(2): 566 - 578. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Riou, R. Saffroy, J. Comoy, M. Gross-Goupil, J.-P. Thiery, J.-F. Emile, D. Azoulay, D. Piatier-Tonneau, A. Lemoine, and B. Debuire Investigation in Liver Tissues and Cell Lines of the Transcription of 13 Genes Mapping to the 16q24 Region That Are Frequently Deleted in Hepatocellular Carcinoma Clin. Cancer Res., October 1, 2002; 8(10): 3178 - 3186. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Okazaki, N. Takahashi, S.-i. Kojima, Y. Masuho, and H. Koga Protocadherin LKC, a new candidate for a tumor suppressor of colon and liver cancers, its association with contact inhibition of cell proliferation Carcinogenesis, July 1, 2002; 23(7): 1139 - 1148. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kajiyama, F. Kikkawa, T. Suzuki, K. Shibata, K. Ino, and S. Mizutani Prolonged Survival and Decreased Invasive Activity Attributable to Dipeptidyl Peptidase IV Overexpression in Ovarian Carcinoma Cancer Res., May 1, 2002; 62(10): 2753 - 2757. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Jhaveri, C. Wagner, and J. B. Trepel Impact of Extracellular Folate Levels on Global Gene Expression Mol. Pharmacol., December 1, 2001; 60(6): 1288 - 1295. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Zhong, Y. Delgado, J. Gomez, S. W. Lee, and R. Perez-Soler Loss of H-Cadherin Protein Expression in Human Non-Small Cell Lung Cancer Is Associated with Tumorigenicity Clin. Cancer Res., June 1, 2001; 7(6): 1683 - 1687. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Marschitz, J. Lechner, I. Mosser, M. Dander, R. Montesano, and H. Schramek Differential expression of cell-cell adhesion proteins and cyclin D in MEK1-transdifferentiated MDCK cells Am J Physiol Cell Physiol, November 1, 2000; 279(5): C1472 - C1482. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Pötter, C. Bergwitz, and G. Brabant The Cadherin-Catenin System: Implications for Growth and Differentiation of Endocrine Tissues Endocr. Rev., April 1, 1999; 20(2): 207 - 239. [Abstract] [Full Text]
|
|