Carcinogenesis, Vol 19, 973-978, Copyright © 1998 by Oxford University Press
T Helleday, C Arnaudeau and D Jenssen
A growing body of carcinogens are known to affect genetic recombination in
mammalian cells and to thereby interfere with the process of
carcinogenesis. In order to screen for recombinogenic effects of chemical
and physical agents a variety of in vitro assay systems utilizing mammalian
cells have been developed. However, the effects of potential carcinogens
differ in these different systems. In order to investigate this phenomenon
further, we have employed two different assay procedures, involving
spontaneous duplication mutants in mammalian cells, which respond to
homologous or non-homologous recombination. Four carcinogens were
investigated, i.e. Aroclor 1221, benzene, methylmethanesulphonate (MMS) and
thiourea, as were gamma- and UV-irradiation. With the exception of thiourea
all of these factors resulted in elevated frequencies of homologous
recombination. On the other hand, only UV-irradiation affected the rate of
non-homologous recombination. These results indicate that substrate length
and/or the recombination mechanism may influence the recombinogenic
response of mammalian fibroblasts to carcinogenic factors. Thus, procedures
for recombinogenic effects of carcinogens should consider the different
pathways of recombination occurring in mammalian cells.
ARTICLES
Effects of carcinogenic agents upon different mechanisms for intragenic recombination in mammalian cells
Department of Genetic and Cellular Toxicology, Wallenberg Laboratory, Stockholm University, Sweden. helleday@genetics.su.se
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