Carcinogenesis, Vol 19, 1257-1262, Copyright © 1998 by Oxford University Press
Q Liu, Y Wang, KA Crist, ZY Wang, YR Lou, MT Huang, AH Conney and M You
In the present study, administration of green tea to SKH-1 mice, via the
drinking fluid, was found to significantly reduce the incidence and volume
of ultraviolet B (UVB) radiation-induced skin tumors. Thirty-six skin
tumors induced by UVB and 32 skin tumors induced by UVB, in mice treated
with green tea in their drinking water, were collected and examined for the
presence of mutations in the p53 gene. Polymerase chain reaction products
from p53 exons 5-8 were screened by single- strand conformation
polymorphism and direct sequence analyses. Eight of 36 UVB-induced tumors
contained nine p53 mutations, with four in exon 5 and five in exon 8. In
contrast, nine of 32 UVB-induced tumors in mice treated with green tea
contained 11 p53 mutations, with two in exon 5, five in exon 6 and four in
exon 8. All of the p53 mutations occurred at dipyrimidine sequences. These
results were further corroborated by p53 immunohistochemistry. The most
frequent mutations were C-->T or T-->C transitions, which are
consistent with the genetic alterations caused by UVB exposure.
Interestingly, mutations found in exon 6 of the p53 gene occurred only in
tumors from the UVB/green tea group. Thus, the tumors observed in
UVB/green-tea-treated mice have a different exon distribution of p53
mutations than tumors obtained from mice treated with UVB alone.
ARTICLES
Effect of green tea on p53 mutation distribution in ultraviolet B radiation-induced mouse skin tumors
Department of Pathology, Medical College of Ohio, Toledo 43614, USA.
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