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Carcinogenesis, Vol. 20, No. 2, 347-350, February 1999
© 1999 Oxford University Press

Short Communications

Pseudoenzymatic reduction of N-hydroxy-2-acetylaminofluorene to 2-acetylaminofluorene mediated by cytochrome P450

Shigeyuki Kitamura1, Koji Takekawa, Kazumi Sugihara, Kiyoshi Tatsumi and Shigeru Ohta

Institute of Pharmaceutical Science, Hiroshima University School of Medicine, Kasumi 1-2-3, Minami-ku, Hiroshima 734-8551, Japan

N-hydroxy-2-acetylaminofluorene (N-OH-AAF) was reduced to 2-acetylaminofluorene by rat liver microsomes in the presence of both NAD(P)H and FAD under anaerobic conditions. The microsomal reduction proceeds as if it were an enzymatic reaction. However, when the microsomes were boiled, the activity was not abolished, but was enhanced. The activity was also observed with cytochrome P450 2B1 alone, without NADPH-cytochrome P450 reductase, in the presence of these cofactors. Hematin also exhibited a significant reducing activity in the presence of both a reduced pyridine nucleotide and FAD. The activities of microsomes, cytochrome P450 2B1 and hematin were also observed upon the addition of photochemically reduced FAD instead of both NAD(P)H and FAD. The microsomal reduction of N-OH-AAF appears to be a non-enzymatic reaction by the reduced flavin, catalyzed by the heme group of cytochrome P450.

Abbreviations: AAF, 2-acetylaminofluorene; N-OH-AAF, N-hydroxy-2-acetylaminofluorene.

1 To whom correspondence should be addressed Email: kitamura{at}pharm.hiroshima-u.ac.jp


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