Carcinogenesis, Vol. 20, No. 6, 1049-1054,
June 1999
© 1999 Oxford University Press
Carcinogenesis |
DNA polymerase ß expression differences in selected human tumors and cell lines
Laboratory of Structural Biology, National Institute of Environmental Health Sciences, PO Box 12233 and
1 Department of Functional Genetics, GlaxoWellcome Inc., Five Moore Drive, Research Triangle Park, NC 27709 and
2 Department of Medicine and Cell Biology, Vanderbilt University, School of Medicine, Nashville, TN 37232, USA
A long-standing question in cancer biology has been the extent to which DNA repair may be altered during the process of carcinogenesis. We have shown recently that DNA polymerase ß (ß-pol) provides a rate-determining function during in vitro repair of abasic sites by one of the mammalian DNA base excision repair pathways. Therefore, altered expression of ß-pol during carcinogenesis could alter base excision repair and, consequently, be critical to the integrity of the mammalian genome. We examined the expression of ß-pol in several cell lines and human adenocarcinomas using a quantitative immunoblotting method. In cell lines from normal breast or colon, the level of ß-pol was ~1 ng/mg cell extract, whereas in all of the breast and colon adenocarcinoma cell lines tested, a higher level of ß-pol was observed. In tissue samples, colon adenocarcinomas had a higher level of ß-pol than adjacent normal mucosa. Breast adenocarcinomas exhibited a wide range of ß-pol expression: one tumor had a much higher level of ß-pol (286 ng/mg cell extract) than adjacent normal breast tissue, whereas another tumor had the same level of ß-pol as adjacent normal tissue. Differences in ß-pol expression level, from normal to elevated, were also observed with prostate adenocarcinomas. All kidney adenocarcinomas tested had a slightly lower ß-pol level than adjacent normal tissue. This study reveals that the base excision repair enzyme DNA polymerase ß is up-regulated in some types of adenocarcinomas and cell lines, but not in others.
Abbreviations: BER, base excision repair; ß-pol, ß-polymerase; PBS, phosphate-buffered saline.
3 To whom correspondence should be addressed Email: wilson5{at}niehs.nih.gov
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