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Carcinogenesis, Vol. 20, No. 7, 1363-1368, July 1999
© 1999 Oxford University Press

Short Communications

Analysis of loss of heterozygosity in neoplastic nodules induced by diethylnitrosamine in the resistant BFF1 rat strain

Manuela Gariboldi, Rosa Pascale1, Giacomo Manenti, Maria R.De Miglio1, Diego Calvisi1, Angelo Carru1, Tommaso A. Dragani and Francesco Feo1,2

Department of Experimental Oncology A, Istituto Nazionale Tumori, Milan, Italy and
1 Department of Biomedical Sciences, Division of Experimental Pathology and Oncology, University of Sassari, Italy

Loss of heterozygosity (LOH) at specific chromosomal regions is a frequent event in poorly differentiated human hepatocellular carcinomas (HCCs), but rare in mouse HCCs. This behavior could depend on interspecies differences in mechanisms of hepatocarcinogenesis or in developmental stage of lesions. To verify if LOH is involved in rat hepatocarcinogenesis, we studied LOH frequency in slowly growing neoplastic nodules induced by Solt–Farber model in diethylnitrosamine-initiated BFF1 rats. We analyzed, with microsatellites, markers at 67 rat loci dispersed over all chromosomes, corresponding to regions homologous to those lost in human HCCs or containing hepatocellular susceptibility (Hcs) or resistance (Hcr) loci in rat and mouse. In agreement with previous findings with mouse HCCs, but at variance with human HCCs, no detectable LOH was found at any locus in rats, suggesting rare LOH involvement in neoplastic nodules, with low tendency to progress to full malignancy, of BFF1 rats.

Abbreviations: BFF1, (BNxF344)F1 hybrid; BN, Brown Norway; F344, Fisher 344; GST-P, placental glutathione S-transferase; HCC, hepatocellular carcinoma; LOH, loss of heterozygosity; SSLP, single sequence length polymorphism.

2 To whom correspondence should be addressed Email: feo{at}ssmain.uniss.it


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