Carcinogenesis, Vol. 20, No. 9, 1845-1853,
September 1999
© 1999 Oxford University Press
Carcinogenesis |
A deficiency in a 230 kDa DNA repair protein in Fanconi anemia complementation group A cells is corrected by the FANCA cDNA
Department of Pathology and Laboratory Medicine, UMDNJ–New Jersey Medical School, 185 South Orange Avenue, Newark, NJ 07103 and the Graduate School of Biomedical Sciences, Newark, NJ and
1 UNC Gene Therapy Center, University of North Carolina, Chapel Hill, NC 27599, USA
Cells from individuals with the cancer-prone, inherited disorder Fanconi anemia (FA) are hypersensitive to DNA interstrand cross-linking agents and this hypersensitivity correlates with a defect in ability to repair this type of damage to their DNA. We have isolated a DNA endonuclease complex from the nuclei of normal human cells which is involved in repair of DNA interstrand cross-links and have shown that in FA complementation group A (FA-A) cells there is a defect in ability of this complex to incise DNA containing interstrand cross-links. In order to identify the specific protein(s) in this complex which is defective in FA-A cells, monoclonal antibodies (mAbs) were developed against proteins in the normal complex. One of these mAbs, which is against a protein with a molecular weight of ~230 kDa, completely inhibited the ability of the normal complex to incise cross-linked DNA. Western blot analysis has shown that there is a deficiency in this protein in FA-A cells. Electophoretic analysis has also indicated that there are reduced levels of this protein in FA-A compared with normal cells. Studies carried out utilizing FA-A cells which have been stably transduced with a retroviral vector expressing the FANCA cDNA have shown that the DNA repair defect in these cells has been corrected; levels of unscheduled DNA synthesis are at least as great as those of normal human cells. In addition, in the transduced cells the deficiency in the 230 kDa protein has been corrected, as determined by both western blot and electrophoretic analysis. These results indicate that the FANCA gene plays a role in the expression or stability of the 230 kDa protein.
Abbreviations: BCIP-NBT, 5-bromo-4-chloro-3-indolyl phosphate and nitroblue tetrazolium; CS, Cockayne syndrome; FA, Fanconi anemia; FA-A, Fanconi anemia complementation group A; mAb, monoclonal antibody; MMC, mitomycin C; 8-MOP, 8-methoxypsoralen; NER, nucleotide excision repair; PBS, phosphate-buffered saline; TMP, 4,5',8-trimethylpsoralen; UDS, unscheduled DNA synthesis.
2 To whom correspondence should be addressed Email: mlambert{at}umdnj.edu
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