Carcinogenesis, Vol. 21, No. 4, 715-725,
April 2000
© 2000 Oxford University Press
Carcinogenesis |
LacI mutation spectra following benzo[a]pyrene treatment of Big Blue® mice
1 Institute for Environmental Studies, Louisiana State University, Baton Rouge, LA 70803, USA,
2 Centre for Environmental Health, University of Victoria, Victoria, BC V8W 2Y2, Canada,
3 Molecular Mutagenesis Group, Laboratory of Environmental Carcinogenesis and Mutagenesis and
4 Biostatistics Branch, National Institute of Environmental Health Sciences, Research Triangle Park, NC 27709, USA
The mutation spectrum of the lacI gene from the liver of C57Bl6 Big Blue® transgenic mice treated with benzo[a]pyrene (B[a]P) has been compared with the spectrum of spontaneous mutations observed in the liver of untreated Big Blue® mice. Mice were treated with B[a]P for 3 days followed by a partial hepatectomy one day after the last injection. Liver tissue was removed for analysis at hepatectomy and, again, 3 days later at the time of sacrifice. Earlier, we reported that the lacI mutant frequency in these B[a]P-treated mice was elevated in the liver both at the time of hepatectomy and at sacrifice; however, a statistically significant increase in the mutant frequency was observed only at sacrifice. In this study, the DNA sequence spectra of lacI mutations observed in the liver of B[a]P-treated Big Blue® mice at hepatectomy and at time of sacrifice were compared with each other and with the spectrum of spontaneous liver mutations. No differences were observed between the two B[a]P-treatment spectra. However, mutation frequencies of both GC
TA and GCCG at the time of hepatectomy and at sacrifice were significantly elevated compared with the spontaneous frequency of these same transversions. Also, the frequency of ATTA transversions was significantly higher than the spontaneous frequency at the time of hepatectomy but not at sacrifice. The frequency of all other classes of mutations scored was not significantly different from the frequency of these same events in the spontaneous spectra. These data support the view that B[a]P treatment results in the induction of GCTA and GCCG transversions within 1 day of the last injection and they provide insights regarding the relative roles of benzo[a]pyrene-7,8-diol-9,10-epoxide and radical cations of B[a]P in B[a]P-induced mutagenesis in vivo. Finally, these data provide evidence for B[a]P-induced mutagenesis under conditions where no statistical increase in mutant frequency could be shown.
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