Carcinogenesis, Vol. 21, No. 6, 1135-1141,
June 2000
© 2000 Oxford University Press
Cancer Biology |
Comparative repair of the endogenous lesions 8-oxo-7,8-dihydroguanine (8-oxoG), uracil and abasic site by mammalian cell extracts: 8-oxoG is poorly repaired by human cell extracts
1 DNA Repair Unit and
2 Mutagenesis Laboratory, Istituto Nazionale Ricerca Cancro, Largo Rosanna Benzi n. 10, 16132 Genova, Italy
The repair of the endogenous lesions 8-oxo-7,8-dihydroguanine (8-oxoG), uracil (U) and natural abasic site (AP site) was investigated using an in vitro base excision repair assay in which a plasmid substrate containing a single lesion at a defined position was repaired by mammalian cell extracts. Repair replication of an 8-oxoG/cytosine base pair performed by normal human cell extracts was ~5-fold less efficient than repair of a U/adenine base pair and, in turn, the latter was repaired ~10-fold less efficiently than an AP site placed in front of an adenine. A similar pattern of repair capacity for the three lesions was observed in Chinese hamster extracts. Repair of 8-oxoG was performed by the one nucleotide insertion pathway only. The lower repair replication ability of 8-oxoG with respect to U was linked to a lower DNA glycosylase (base removal) activity rather than to inability to process the ß-elimination cleaved strand left by the AP lyase activity associated with human oxoguanine DNA glycosylase 1. The data show that DNA repair of 8-oxoG is poor in human cells in comparison with other frequent endogenous lesions.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. G. Kozmin, Y. Sedletska, A. Reynaud-Angelin, D. Gasparutto, and E. Sage The formation of double-strand breaks at multiply damaged sites is driven by the kinetics of excision/incision at base damage in eukaryotic cells Nucleic Acids Res., April 1, 2009; 37(6): 1767 - 1777. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. S. Cooke, R. Olinski, S. Loft, and members of the European Standards Committee on Uri Measurement and Meaning of Oxidatively Modified DNA Lesions in Urine Cancer Epidemiol. Biomarkers Prev., January 1, 2008; 17(1): 3 - 14. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Eot-Houllier, M. Gonera, D. Gasparutto, C. Giustranti, and E. Sage Interplay between DNA N-glycosylases/AP lyases at multiply damaged sites and biological consequences Nucleic Acids Res., May 11, 2007; 35(10): 3355 - 3366. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. J. Raffoul, D. C. Cabelof, J. Nakamura, L. B. Meira, E. C. Friedberg, and A. R. Heydari Apurinic/Apyrimidinic Endonuclease (APE/REF-1) Haploinsufficient Mice Display Tissue-specific Differences in DNA Polymerase {beta}-Dependent Base Excision Repair J. Biol. Chem., April 30, 2004; 279(18): 18425 - 18433. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A.-K. Olsen, N. Duale, M. Bjoras, C. T. Larsen, R. Wiger, J. A. Holme, E. C. Seeberg, and G. Brunborg Limited repair of 8-hydroxy-7,8-dihydroguanine residues in human testicular cells Nucleic Acids Res., February 15, 2003; 31(4): 1351 - 1363. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Vens, E. Dahmen-Mooren, M. Verwijs-Janssen, W. Blyweert, L. Graversen, H. Bartelink, and A. C. Begg The role of DNA polymerase {beta} in determining sensitivity to ionizing radiation in human tumor cells Nucleic Acids Res., July 1, 2002; 30(13): 2995 - 3004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. A. Sokhansanj, G. R. Rodrigue, J. P. Fitch, and D. M. W. III A quantitative model of human DNA base excision repair. I. mechanistic insights Nucleic Acids Res., April 15, 2002; 30(8): 1817 - 1825. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Mambo, S. G. Nyaga, V. A. Bohr, and M. K. Evans Defective Repair of 8-Hydroxyguanine in Mitochondria of MCF-7 and MDA-MB-468 Human Breast Cancer Cell Lines Cancer Res., March 1, 2002; 62(5): 1349 - 1355. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Frosina Counteracting spontaneous transformation via overexpression of rate-limiting DNA base excision repair enzymes Carcinogenesis, September 1, 2001; 22(9): 1335 - 1341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Cappelli, T. Hazra, J. W. Hill, G. Slupphaug, M. Bogliolo, and G. Frosina Rates of base excision repair are not solely dependent on levels of initiating enzymes Carcinogenesis, March 1, 2001; 22(3): 387 - 393. [Abstract] [Full Text] [PDF]
|
|