Carcinogenesis, Vol. 21, No. 7, 1453-1456,
July 2000
© 2000 Oxford University Press
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APC truncation and increased ß-catenin levels in a human breast cancer cell line
1 Department of Pathology and
2 Department of Obstetrics and Gynecology, Columbia University, College of Physicians and Surgeons, 630 West 168th Street, New York, NY 10032 and
3 Department of Pathology, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021, USA
Mutations in the Adenomatous Polyposis Coli (APC) tumor suppressor gene or the ß-catenin gene are present in most colon cancers and less frequently in other tumor types. In this study, we screened 24 human breast cancer cell lines and three immortalized human breast epithelial cell lines for alterations in ß- and 
-catenin and APC by western blotting, protein truncation assay and DNA sequence analysis. In one cell line (DU 4475), an APC mutation was identified (E1577stop) that resulted in expression of truncated APC. This mutation was associated with elevated cytosolic ß-catenin levels, probably due to loss of APC function, as in colon cancers. No mutations were found in exon 3 of the ß- or -catenin genes. We conclude that APC mutations and ß-catenin upregulation may occur with low frequency in human breast cancer cells.
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