Carcinogenesis, Vol. 22, No. 10, 1633-1639,
October 2001
© 2001 Oxford University Press
CANCER BIOLOGY |
Bcl-2 overexpression attenuates resveratrol-induced apoptosis in U937 cells by inhibition of caspase-3 activity
Department of Immunology,
1 Department of Microbiology, School of Medicine, Keimyung University, 194 DongSan-Dong Jung-Gu, Taegu, 700-712,
2 Department of Microbiology, College of Natural Sciences, Kyungpook University, Taegu,
3 Department of Physiology, College of Medicine, The Catholic University of Korea, Seoul, 137-701,
4 Department of Life Science, Daejin University, Pochon-gun, Kyeonggido,
5 Department of Biochemistry, College of Medicine, Yeungnam University, Taegu, South Korea and
6 Department of Pathology, Greenebaum Cancer Center, University of Maryland Baltimore, Maryland, USA
Resveratrol has been shown to induce anti-proliferation and apoptosis of human cancer cell lines. In the present study, we determined the effect of high intracellular levels of the anti-apoptosis protein Bcl-2 on caspase-3 activation, PLC-
1 degradation and cytochrome c release during resveratrol-induced apoptosis. For this, we used U937/vector and U937/Bcl-2 cells, which were generated by transfection of the cDNA of the Bcl-2 gene. As compared with U937/vector, U937/Bcl-2 cells exhibited a 4-fold greater expression of Bcl-2. Treatment with 60 or 100 µM resveratrol for 24 h produced morphological features of apoptosis and DNA fragmentation in U937/vector cells, respectively. This was associated with caspase-3 activation and PLC-1 degradation. In contrast, resveratrol-induced caspase-3 activation and PLC-1 degradation and apoptosis were significantly inhibited in U937/Bcl-2 cells. Bcl-2 overexpressing cells exhibited less cytochrome c release and sustained expression levels of the IAP proteins during resveratrol-induced apoptosis. In addition, these findings indicate that Bcl-2 inhibits resveratrol-induced apoptosis by a mechanism that interferes with cytochrome c release and activity of caspase-3 that is involved in the execution of apoptosis.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. C. Kowalczyk, Z. Walaszek, P. Kowalczyk, T. Kinjo, M. Hanausek, and T. J. Slaga Differential effects of several phytochemicals and their derivatives on murine keratinocytes in vitro and in vivo: implications for skin cancer prevention Carcinogenesis, June 1, 2009; 30(6): 1008 - 1015. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Singletary and J. Milner Diet, Autophagy, and Cancer: A Review Cancer Epidemiol. Biomarkers Prev., July 1, 2008; 17(7): 1596 - 1610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. F. Trincheri, G. Nicotra, C. Follo, R. Castino, and C. Isidoro Resveratrol induces cell death in colorectal cancer cells by a novel pathway involving lysosomal cathepsin D Carcinogenesis, May 1, 2007; 28(5): 922 - 931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E.-O. Lee, H.-J. Lee, H.-S. Hwang, K.-S. Ahn, C. Chae, K.-S. Kang, J. Lu, and S.-H. Kim Potent inhibition of Lewis lung cancer growth by heyneanol A from the roots of Vitis amurensis through apoptotic and anti-angiogenic activities Carcinogenesis, October 1, 2006; 27(10): 2059 - 2069. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Jiang, L. Zhang, J. Kuo, K. Kuo, S. C. Gautam, L. Groc, A. I. Rodriguez, D. Koubi, T. Jackson Hunter, G. B. Corcoran, et al. Resveratrol-induced apoptotic death in human U251 glioma cells Mol. Cancer Ther., April 1, 2005; 4(4): 554 - 561. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. W. Opipari Jr., L. Tan, A. E. Boitano, D. R. Sorenson, A. Aurora, and J. R. Liu Resveratrol-induced Autophagocytosis in Ovarian Cancer Cells Cancer Res., January 15, 2004; 64(2): 696 - 703. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Delmas, C. Rebe, S. Lacour, R. Filomenko, A. Athias, P. Gambert, M. Cherkaoui-Malki, B. Jannin, L. Dubrez-Daloz, N. Latruffe, et al. Resveratrol-induced Apoptosis Is Associated with Fas Redistribution in the Rafts and the Formation of a Death-inducing Signaling Complex in Colon Cancer Cells J. Biol. Chem., October 17, 2003; 278(42): 41482 - 41490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. D. Ragione, V. Cucciolla, V. Criniti, S. Indaco, A. Borriello, and V. Zappia p21Cip1 Gene Expression Is Modulated by Egr1: A NOVEL REGULATORY MECHANISM INVOLVED IN THE RESVERATROL ANTIPROLIFERATIVE EFFECT J. Biol. Chem., June 20, 2003; 278(26): 23360 - 23368. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Calviello, F. Di Nicuolo, E. Piccioni, M.E. Marcocci, S. Serini, N. Maggiano, K. H. Jones, David. G. Cornwell, and P. Palozza {gamma}-Tocopheryl quinone induces apoptosis in cancer cells via caspase-9 activation and cytochrome c release Carcinogenesis, March 1, 2003; 24(3): 427 - 433. [Abstract] [Full Text] [PDF]
|
|