Carcinogenesis, Vol. 22, No. 10, 1721-1725,
October 2001
© 2001 Oxford University Press
CARCINOGENESIS |
Intestinal microflora plays a crucial role in the genotoxicity of the cooked food mutagen 2-amino-3-methylimidazo[4,5-f]quinoline (IQ)
Institute of Cancer Research, Borschkegasse 8a, A-1090 Vienna, Austria,
1 Institute of Environmental Hygiene, University of Vienna, Kinderspitalgasse 15, A-1090 Vienna, Austria and
2 National Institute for Agricultural Research (INRA), Unit on Ecology and Physiology of the Digestive Tract, Jouy-en Josas, France
We investigated the impact of the intestinal microflora on the genotoxicity of 2-amino-3-methylimidazo[4,5-f] quinoline (IQ), a mutagenic/carcinogenic heterocyclic amine commonly found in fried meats and fish. In parallel, we also examined the effect of the microflora on the protective effect of glucotropaeolin (GT), a glucosinolate contained in cruciferous vegetables, towards IQ-induced genotoxic effect. Conventional (NF), human flora associated (HFA) and germ free (GF) rats were treated either with 90 mg/kg IQ alone, 150 mg/kg GT alone or a combination of the two by gavage and DNA damage was determined in liver and colon cells using the alkaline single cell gel electrophoresis (SCGE) or comet assay. IQ caused a significant effect in both organs of all groups. However, DNA damage was most pronounced in NF animals. In colon cells, DNA migration was 6-fold more in IQ-exposed rats as compared with untreated controls. The effect measured with liver cells was similar. In comparison to NF rats, in HFA rats, tail length of the comets was 22 and 53% lower in liver and colon cells, respectively. Significantly weaker effects were seen in GF animals (66 and 75% lower damage in hepatocytes and colonocytes, respectively, than in NF animals). Pretreatment with GT led to a complete reduction of IQ-induced DNA damage regardless of the microbial status of the animals. In addition, a moderate decrease in spontaneous DNA damage was seen in animals that received GT alone. Our results show that the microflora has a strong impact on the genotoxic effects of IQ. We conclude that the alkaline SCGE assay with rats harbouring different flora opens new possibilities to investigate the role of intestinal bacteria on health risks caused by dietary carcinogens.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Rohrmann, S. Hermann, and J. Linseisen Heterocyclic aromatic amine intake increases colorectal adenoma risk: findings from a prospective European cohort study Am. J. Clinical Nutrition, May 1, 2009; 89(5): 1418 - 1424. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Vanhaecke, F. Vercruysse, N. Boon, W. Verstraete, I. Cleenwerck, M. De Wachter, P. De Vos, and T. van de Wiele Isolation and Characterization of Human Intestinal Bacteria Capable of Transforming the Dietary Carcinogen 2-Amino-1-Methyl-6-Phenylimidazo[4,5-b]Pyridine Appl. Envir. Microbiol., March 1, 2008; 74(5): 1469 - 1477. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Humblot, M. Murkovic, L. Rigottier-Gois, M. Bensaada, A. Bouclet, C. Andrieux, J. Anba, and S. Rabot {beta}-Glucuronidase in human intestinal microbiota is necessary for the colonic genotoxicity of the food-borne carcinogen 2-amino-3-methylimidazo[4,5-f]quinoline in rats Carcinogenesis, November 1, 2007; 28(11): 2419 - 2425. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Humblot, B. Combourieu, M.-L. Vaisanen, J.-P. Furet, A.-M. Delort, and S. Rabot 1H Nuclear Magnetic Resonance Spectroscopy-Based Studies of the Metabolism of Food-Borne Carcinogen 2-Amino-3-Methylimidazo[4,5-f]Quinoline by Human Intestinal Microbiota Appl. Envir. Microbiol., September 1, 2005; 71(9): 5116 - 5123. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Holmes and J. Nicholson Variation in Gut Microbiota Strongly Influences Individual Rodent Phenotypes Toxicol. Sci., September 1, 2005; 87(1): 1 - 2. [Full Text] [PDF]
|
|
|
|
 |
|
 M. M. Huycke and H. R. Gaskins Commensal Bacteria, Redox Stress, and Colorectal Cancer: Mechanisms and Models Experimental Biology and Medicine, July 1, 2004; 229(7): 586 - 597. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Zsivkovits, K. Fekadu, G. Sontag, U. Nabinger, W. W. Huber, M. Kundi, A. Chakraborty, H. Foissy, and S. Knasmuller Prevention of heterocyclic amine-induced DNA damage in colon and liver of rats by different lactobacillus strains Carcinogenesis, December 1, 2003; 24(12): 1913 - 1918. [Abstract] [Full Text] [PDF]
|
|