Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (32)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Wong, B. C. Y.
Right arrow Articles by Lam, S. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wong, B. C. Y.
Right arrow Articles by Lam, S. K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Carcinogenesis, Vol. 22, No. 9, 1349-1354, September 2001
© 2001 Oxford University Press

CANCER BIOLOGY

12-Lipoxygenase inhibition induced apoptosis in human gastric cancer cells

Benjamin Chun Yu Wong3, Wei Ping Wang, Chi Hin Cho1, Xiao Ming Fan, Marie Chia Mi Lin2, Hsiang Fu Kung2 and Shiu Kum Lam

Department of Medicine,
1 Department of Pharmacology and
2 Institute of Molecular Biology, University of Hong Kong, Hong Kong, China

Arachidonic acid release from membrane phospholipids is essential for tumour cell proliferation. Lipoxygenases constitute a pathway for arachidonate metabolism. The present study investigated the expression of 12-lipoxygenase and its effect on cell proliferation as well as survival in two human gastric cancer cell lines (AGS and MKN-28). RT–PCR and western blots, respectively, showed 12-LOX mRNA and protein expression in both AGS and MKN-28 cell lines. Treatment with a 12-LOX inhibitor, baicalein, significantly inhibited cancer cell proliferation, but a metabolite of 12-LOX activity, 12 hydroxyeicosatetraenoic acid (12-HETE) reversed baicalein-induced growth inhibition. Furthermore, the blockade of the 12-LOX pathway through a 12-LOX inhibitor and antisense induced apoptosis of gastric cancer cell lines. The biochemical characteristics of apoptosis were p53-independent combined with a decrease in bcl-2 expression. Caspase-7 was proteolytically activated and responsible for the apoptosis execution.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
H. Taniguchi, T. Yoshida, M. Horinaka, T. Yasuda, A. E. Goda, M. Konishi, M. Wakada, K. Kataoka, T. Yoshikawa, and T. Sakai
Baicalein Overcomes Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand Resistance via Two Different Cell-Specific Pathways in Cancer Cells but not in Normal Cells
Cancer Res., November 1, 2008; 68(21): 8918 - 8927.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
Y.-S. Piao, Y.-C. Du, H. Oshima, J.-C. Jin, M. Nomura, T. Yoshimoto, and M. Oshima
Platelet-type 12-lipoxygenase accelerates tumor promotion of mouse epidermal cells through enhancement of cloning efficiency
Carcinogenesis, February 1, 2008; 29(2): 440 - 447.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
A. M. Monjazeb, K. P. High, A. Connoy, L. S. Hart, C. Koumenis, and F. H. Chilton
Arachidonic acid-induced gene expression in colon cancer cells
Carcinogenesis, October 1, 2006; 27(10): 1950 - 1960.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
M. Bonham, J. Posakony, I. Coleman, B. Montgomery, J. Simon, and P. S. Nelson
Characterization of Chemical Constituents in Scutellaria baicalensis with Antiandrogenic and Growth-Inhibitory Activities toward Prostate Carcinoma
Clin. Cancer Res., May 15, 2005; 11(10): 3905 - 3914.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
K. Suk, H. Lee, S. S. Kang, G. J. Cho, and W. S. Choi
Flavonoid Baicalein Attenuates Activation-Induced Cell Death of Brain Microglia
J. Pharmacol. Exp. Ther., May 1, 2003; 305(2): 638 - 645.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
J. Wu, H. H. X. Xia, S. P. Tu, D. M. Fan, M. C. M. Lin, H. F. Kung, S. K. Lam, and B. C. Y. Wong
15-Lipoxygenase-1 mediates cyclooxygenase-2 inhibitor-induced apoptosis in gastric cancer
Carcinogenesis, February 1, 2003; 24(2): 243 - 247.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
P. E. Lovat, S. Oliverio, M. Ranalli, M. Corazzari, C. Rodolfo, F. Bernassola, K. Aughton, M. Maccarrone, Q. D. C. Hewson, A. D. J. Pearson, et al.
GADD153 and 12-Lipoxygenase Mediate Fenretinide-induced Apoptosis of Neuroblastoma
Cancer Res., September 15, 2002; 62(18): 5158 - 5167.
[Abstract] [Full Text] [PDF]

Home page
 J. Med. Genet.Home page
W J Lemon, H Bernert, H Sun, Y Wang, and M You
Identification of candidate lung cancer susceptibility genes in mouse using oligonucleotide arrays
J. Med. Genet., September 1, 2002; 39(9): 644 - 655.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
Graham. P. Pidgeon, M. Kandouz, A. Meram, and K. V. Honn
Mechanisms Controlling Cell Cycle Arrest and Induction of Apoptosis after 12-Lipoxygenase Inhibition in Prostate Cancer Cells
Cancer Res., May 1, 2002; 62(9): 2721 - 2727.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.