Carcinogenesis, Vol. 22, No. 9, 1421-1427,
September 2001
© 2001 Oxford University Press
MOLECULAR EPIDEMIOLOGY AND CANCER PREVENTION |
Inhibition of phorbol ester-induced AP-1–DNA binding, c-Jun protein and c-jun mRNA by dietary energy restriction is reversed by adrenalectomy in SENCAR mouse epidermis
1 Department of Food Science and Human Nutrition, Iowa State University, Ames, IA 50011-1061 and
2 Eppley Institute for Research in Cancer and Allied Diseases and
3 Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, NE, 68198-6805, USA
4 Joint first authors
The aim of this study was to determine the effects of 40% dietary energy restriction (DER) relative to ad libitum feeding on AP-1–DNA binding and expression of c-Jun protein and c-jun mRNA in SENCAR mouse skin treated with acetone or 12-O-tetradecanoylphorbol 13-acetate (TPA). The role of the glucocorticoid hormone corticosterone (CCS) was investigated by adding CCS or vehicle control to the drinking water of adrenalectomized mice. AP-1–DNA binding, measured by electrophoretic mobility shift assay, showed that TPA treatment for 4 h increased AP-1–DNA binding by 2-fold over acetone controls (P < 0.05) and that DER reduced basal and TPA-induced AP-1–DNA binding in comparison with ad libitum fed groups in sham-operated mice (P < 0.05). TPA treatment increased c-Jun protein levels in control fed mice (4-fold) and in DER mice (2-fold) over basal levels 4 h post-treatment (P < 0.05). Analyzed over all groups, DER reduced c-Jun protein levels (P < 0.01) and this effect was reversed by adrenalectomy. TPA induction of c-jun mRNA was also reduced by DER compared with ad libitum fed mice (P < 0.05). Adrenalectomy and CCS supplementation demonstrated that the effects of DER on AP-1–DNA binding were mediated in part by CCS. Measurement of blood plasma CCS concentrations showed that: (i) DER increased CCS 5-fold over ad libitum fed mice in sham-operated animals (P < 0.05); (ii) adrenalectomy decreased CCS over sham-operated mice (P < 0.05); (iii) TPA treatment had no effect on CCS. Blood plasma IGF-I concentrations were unaffected by CCS modulation or TPA treatment but were decreased by DER compared with ad libitum fed mice (P < 0.05). Thus, dietary energy restriction may inhibit cancer mechanistically by reducing overall AP-1 transcription through a process that is mediated in part by glucocorticoid hormones.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L. Xie, Y. Jiang, P. Ouyang, J. Chen, H. Doan, B. Herndon, J. E. Sylvester, K. Zhang, A. Molteni, M. Reichle, et al. Effects of Dietary Calorie Restriction or Exercise on the PI3K and Ras Signaling Pathways in the Skin of Mice J. Biol. Chem., September 21, 2007; 282(38): 28025 - 28035. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Lu, L. Xie, J. Sylvester, J. Wang, J. Bai, R. Baybutt, and W. Wang Different Gene Expression of Skin Tissues Between Mice with Weight Controlled by Either Calorie Restriction or Physical Exercise Exp Biol Med, April 1, 2007; 232(4): 473 - 480. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. W. Stewart, K. Koehler, W. Jackson, J. Hawley, W. Wang, A. Au, R. Myers, and D. F. Birt Prevention of mouse skin tumor promotion by dietary energy restriction requires an intact adrenal gland and glucocorticoid supplementation restores inhibition Carcinogenesis, June 1, 2005; 26(6): 1077 - 1084. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K.-S. Chun, S.-H. Kim, Y.-S. Song, and Y.-J. Surh Celecoxib inhibits phorbol ester-induced expression of COX-2 and activation of AP-1 and p38 MAP kinase in mouse skin Carcinogenesis, May 1, 2004; 25(5): 713 - 722. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. B. Willis and N. H. Colburn Molecular Targets for Cancer Prevention: A Meeting Review of the Third American Cancer Society-Schilling Research Conference Cancer Epidemiol. Biomarkers Prev., October 1, 2002; 11(10): 972 - 978. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Lu, F. Zhang, A. Gupta, and J. Liu Blockade of AP1 Transactivation Abrogates the Abnormal Expression of Breast Cancer-specific Gene 1 in Breast Cancer Cells J. Biol. Chem., August 23, 2002; 277(35): 31364 - 31372. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. J. Thompson, Z. Zhu, and W. Jiang Protection against Cancer by Energy Restriction: All Experimental Approaches Are Not Equal J. Nutr., May 1, 2002; 132(5): 1047 - 1049. [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu, W. Wang, J. Hawley, and D. F. Birt Adrenalectomy Abrogates Reduction of 12-O-Tetradecanoylphorbol-13-acetate-induced Extracellular Signal-regulated Protein Kinase Activity in the Epidermis of Dietary Energy-restricted SENCAR Mice: Implications of Glucocorticoid Hormone Cancer Epidemiol. Biomarkers Prev., March 1, 2002; 11(3): 299 - 304. [Abstract] [Full Text] [PDF]
|
|