Carcinogenesis, Vol. 23, No. 12, 2129-2133,
December 2002
© 2002 Oxford University Press
CARCINOGENESIS |
Comparative analysis of baseline 8-oxo-7,8-dihydroguanine in mammalian cell DNA, by different methods in different laboratories: an approach to consensus
Escodd (European Standards Committee On Oxidative Dna Damage)*
Corresponding author: Professor Andrew Collins, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK Email: a.collins{at}rri.sari.ac.uk
The European Standards Committee on Oxidative DNA Damage (ESCODD) was set up to resolve the problems associated with the measurement of background levels of oxidative DNA damage (in particular 8-oxo-7,8-dihydroguanine, or 8-oxoGua) in human cells. A tendency for DNA oxidation to occur during sample preparation prior to chromatography has been recognized as the source of a very substantial artefact. To assess the success of attempts to eliminate the artefact, ESCODD has distributed to its members standard samples of pig liver and HeLa cells for analysis. Estimates of 8-oxoGua in pig liver, using chromatographic techniques, ranged from 2.23 to 441 per 106 guanines, with a median of 10.47 per 106 guanines. Chromatographic analysis of HeLa cell DNA gave a range of 1.84 to 214 per 106 guanines with a median of 5.23 per 106 guanines. HeLa cell DNA was also analysed by an enzymic approach, in which whole cell DNA was treated with formamidopyrimidine DNA glycosylase, which nicks DNA at sites of 8-oxoGua, and the breaks measured with the comet assay, alkaline unwinding or alkaline elution. Values with these methods ranged from 0.06 to 4.988-oxoGua per 106 guanines, with a median of 0.79 per 106 guanines. Although there are clearly still serious discrepancies between methods and laboratories, the lowest estimates by chromatography (arguably those in which the artefact was best controlled) are only 2.5 times higher than the median value obtained with the enzymic approach.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. Dusinska and A. R. Collins The comet assay in human biomonitoring: gene-environment interactions Mutagenesis, May 1, 2008; 23(3): 191 - 205. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Trapp, M. Schwarz, and B. Epe The Peroxisome Proliferator WY-14,643 Promotes Hepatocarcinogenesis Caused by Endogenously Generated Oxidative DNA Base Modifications in Repair-Deficient Csbm/m/Ogg1-/- Mice Cancer Res., June 1, 2007; 67(11): 5156 - 5161. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. K. Folkmann, S. Loft, and P. Moller Oxidatively damaged DNA in aging dyslipidemic ApoE-/- and wild-type mice Mutagenesis, March 1, 2007; 22(2): 105 - 110. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. R. Ferguson, M. Philpott, and N. Karunasinghe Oxidative DNA Damage and Repair: Significance and Biomarkers J. Nutr., October 1, 2006; 136(10): 2687S - 2689S. [Full Text] [PDF]
|
|
|
|
 |
|
 A. M. Hays, D. Srinivasan, M. L. Witten, D. E. Carter, and R. C. Lantz Arsenic and Cigarette Smoke Synergistically Increase DNA Oxidation in the Lung Toxicol Pathol, June 1, 2006; 34(4): 396 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y.-J. Liu, P.-L. Huang, Y.-F. Chang, Y.-H. Chen, Y.-H. Chiou, Z.-L. Xu, and R.-H. Wong GSTP1 Genetic Polymorphism Is Associated with a Higher Risk of DNA Damage in Pesticide-Exposed Fruit Growers. Cancer Epidemiol. Biomarkers Prev., April 1, 2006; 15(4): 659 - 666. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Tanaka, N. Nakagawa, S. Kuramitsu, S. Yokoyama, and R. Masui Novel Reaction Mechanism of GTP Cyclohydrolase I. High-Resolution X-Ray Crystallography of Thermus thermophilus HB8 Enzyme Complexed with a Transition State Analogue, the 8-Oxoguanine Derivative J. Biochem., September 1, 2005; 138(3): 263 - 275. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R Collins Assays for oxidative stress and antioxidant status: applications to research into the biological effectiveness of polyphenols Am. J. Clinical Nutrition, January 1, 2005; 81(1): 261S - 267S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Rusyn, S. Asakura, B. Pachkowski, B. U. Bradford, M. F. Denissenko, J. M. Peters, S. M. Holland, J. K. Reddy, M. L. Cunningham, and J. A. Swenberg Expression of Base Excision DNA Repair Genes Is a Sensitive Biomarker for in Vivo Detection of Chemical-induced Chronic Oxidative Stress: Identification of the Molecular Source of Radicals Responsible for DNA Damage by Peroxisome Proliferators Cancer Res., February 1, 2004; 64(3): 1050 - 1057. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
U. Nair, H. Bartsch, and J. Nair Prevention of degenerative diseases; clues from studies investigating oxidative stress, Brussels, 13 November 2002 Mutagenesis, September 1, 2003; 18(5): 477 - 483. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Loft, T. Kold-Jensen, N. H. Hjollund, A. Giwercman, J. Gyllemborg, E. Ernst, J. Olsen, T. Scheike, H. E. Poulsen, and J. P. Bonde Oxidative DNA damage in human sperm influences time to pregnancy Hum. Reprod., June 1, 2003; 18(6): 1265 - 1272. [Abstract] [Full Text] [PDF]
|
|