Carcinogenesis, Vol. 23, No. 6, 1039-1046,
June 2002
© 2002 Oxford University Press
CARCINOGENESIS |
Low amounts of the DNA repair XPA protein are sufficient to recover UV-resistance
1 Departamento de Microbiologia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brazil,
2 Instituto de Pesquisas Energéticas e Nucleares (IPEN), Comissão Nacional de Energia Nuclear (CNEN/SP), Supervisão de Radiobiologia, São Paulo, SP, Brazil,
3 Departamento de Anatomia, Instituto de Ciências Biomédicas, Universidade de São Paulo, SP, Brazil and
4 Departamento de Imunologia, Instituto de Ciências Biomédicas, Universidade de São Paulo e Instituto de Investigacião em Imunologia, Instituto do Milênio, SP, Brazil
DNA integrity is threatened by the damaging effects of physical and chemical agents that can affect its function. Nucleotide excision repair (NER) is one of the most known and flexible mechanisms of DNA repair. This mechanism can recognize and remove damages causing DNA double-helix distortion, including the cyclobutane pyrimidine dimers (CPDs) and the pyrimidine-pyrimidone (6–4) photoproducts, promoted by ultraviolet light (UV). The human syndrome xeroderma pigmentosum (XP) is clinically characterized chiefly by the early onset of severe photosensitivity of the exposed regions of the skin, a very high incidence of skin cancers and frequent neurological abnormalities. The xpa gene seems to be involved during UV damage recognition, in both global genome repair (GGR) and transcription-coupled repair (TCR). The modulation of xpa expression may modify the DNA repair rate in the cell genome, providing a valuable contribution to an understanding of the NER process. The controlled expression of the cDNA xpa in XP12RO deficient cells was achieved through the transfection of a muristerone-A inducible vector, pINXA. The INXA15 clone shows good induction of the XPA protein and total complementation of XP12RO cell deficiency. Overexpression of this protein resulted in UV cell survival comparable to normal control human cells. Moreover, low expression of the XPA protein in these cells is sufficient for total complementation in cellular UV sensitivity and DNA repair activity. These data demonstrate that XPA protein concentration is not a limiting factor for DNA repair.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Huard, R. T. Elder, D. Liang, G. Li, and R. Y. Zhao Human Immunodeficiency Virus Type 1 Vpr Induces Cell Cycle G2 Arrest through Srk1/MK2-Mediated Phosphorylation of Cdc25 J. Virol., March 15, 2008; 82(6): 2904 - 2917. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. G. Armelini, K. M. Lima-Bessa, M. C. N. Marchetto, A. R. Muotri, V. Chigancas, R. A. Leite, H. Carvalho, and C. F. M. Menck Exploring DNA damage responses in human cells with recombinant adenoviral vectors Human and Experimental Toxicology, November 1, 2007; 26(11): 899 - 906. [Abstract] [PDF]
|
|
|
|
 |
|
 J. E. Adair, S. C. Maloney, G. A. Dement, K. J. Wertzler, M. J. Smerdon, and R. Reeves High-Mobility Group A1 Proteins Inhibit Expression of Nucleotide Excision Repair Factor Xeroderma Pigmentosum Group A Cancer Res., July 1, 2007; 67(13): 6044 - 6052. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S.F. Biard, E. Despras, A. Sarasin, and J. F. Angulo Development of New EBV-Based Vectors for Stable Expression of Small Interfering RNA to Mimick Human Syndromes: Application to NER Gene Silencing Mol. Cancer Res., September 1, 2005; 3(9): 519 - 529. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Wu, W. Fan, S. Xu, and Y. Zhou Sensitization to the Cytotoxicity of Cisplatin by Transfection with Nucleotide Excision Repair Gene Xeroderma Pigmentosun Group A Antisense RNA in Human Lung Adenocarcinoma Cells Clin. Cancer Res., December 1, 2003; 9(16): 5874 - 5879. [Abstract] [Full Text] [PDF]
|
|