Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (6)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Kim, B. J.
Right arrow Articles by Jung, Y.-K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, B. J.
Right arrow Articles by Jung, Y.-K.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Carcinogenesis, Vol. 23, No. 9, 1411-1417, September 2002
© 2002 Oxford University Press

CANCER BIOLOGY

Sensitizing effects of cadmium on TNF-{alpha}- and TRAIL-mediated apoptosis of NIH3T3 cells with distinct expression patterns of p53

Byung Ju Kim1, Mi-Suk Kim1, Ki-Bae Kim1, Ki-Woo Kim1, Yeon-Mi Hong1, In-Ki Kim1, Han-Woong Lee2 and Yong-Keun Jung1,3

1 Department of Life Science, Kwangju Institute of Science and Technology, 1 Oryong-dong Puk-gu, Kwangju 500-712, Korea and
2 Department of Molecular Cell Biology, Sungkyunkwan University School of Medicine, Suwon 440-746, Korea

Tumor necrosis factor (TNF)-{alpha} and TNF-related apoptosis inducing ligand (TRAIL) share a common signaling pathway. Here we show a novel potentiating effect of cadmium on TNF-{alpha}- or TRAIL-mediated cell death via distinct signaling. TNF-{alpha} or TRAIL sensitized otherwise resistant NIH3T3 embryo fibroblast cells to death, when exposed to cadmium. The potentiating effects elicited by TNF-{alpha} or TRAIL on cell death were NF-{kappa}B- and SAPK/JNK-independent and were not diminished by the expression of Bcl-2. TNF-{alpha} potentiated the cadmium-induced accumulation of p53 but did not affect expression levels of Bax, Mdm2 and p21WAF/CIP. A similar pattern of p53 accumulation was also observed in Balbc/3T3 fibroblasts but not in human tumor cell lines, MCF7 and HeLa cells. The synergistic cell death evoked by TNF-{alpha} and cadmium was attenuated by transient expression of a dominant negative p53Val135 mutant in NIH3T3 cells and was not observed in p53(–/–) mouse embryo fibroblasts, indicating that p53 accumulation appears to contribute to cell death. In contrast, TRAIL did not further increase the cadmium-induced accumulation of p53 despite its potentiation effects on the cadmium-induced cell death. Expression of p53Val135 mutant did not reduce TRAIL- and cadmium-mediated cell death. Taken together, these results suggest that TNF-{alpha} and TRAIL potentiate the cadmium-mediated cell death via distinct p53 expression patterns.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. Amini, M. Saunders, K. Kelley, K. Khalili, and B. E. Sawaya
Interplay between HIV-1 Vpr and Sp1 Modulates p21WAF1 Gene Expression in Human Astrocytes
J. Biol. Chem., October 29, 2004; 279(44): 46046 - 46056.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.