Carcinogenesis Advance Access originally published online on March 28, 2003
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Carcinogenesis, Vol. 24, No. 5, 827-834,
May 2003
© 2003 Oxford University Press
CANCER BIOLOGY |
AMPK-ß1 subunit is a p53-independent stress responsive protein that inhibits tumor cell growth upon forced expression
1 Cancer Molecular Sciences, Pfizer Global Research and Development, Ann Arbor Laboratories, 2800 Plymouth Road, Ann Arbor, MI 48105, USA
2 Department of Radiation Oncology, University of Michigan, Ann Arbor MI 48109, USA
3 To whom correspondence should be addressed Email: yi.sun{at}pfizer.com
In an effort to search for genes responsible for cell growth arrest and/or apoptosis associated with p53 signaling pathways, we profiled a human lung carcinoma line H1299, expressing a temperature-sensitive p53 (V138) against Affymetric human U95Av2 GeneChip A, consisting of 12 000 genes. 133 genes were identified that were either induced or repressed in response to p53-dependent cell growth arrest and apoptotic conditions. Among them, the ß1 subunit, but not other subunits of the AMP-activated protein kinase (AMPK) was strongly induced. The p53 consensus binding site search in the AMPK-ß1 promoter and the first intron identified four such putative sites. However, p53 failed to bind to any of these sites as assayed by in vitro gel retardation and in vivo chromatin immunoprecipitation. Furthermore, northern analysis showed that induction of this gene is independent of p53, as increased expression of the gene was observed in p53 null H1299/Neo control cells when the temperature was shifted to 32°C. Moreover, a DNA damaging agent, etoposide, also induced ß1 subunit expression in multiple human tumor cells, regardless of p53 status. Thus, the ß1 subunit of AMPK is not a p53 downstream target gene, but can be induced by cold shock or the chemotherapeutic drug, etoposide in a p53-independent manner. To determine the biological significance of AMPK-ß1 induction, we over-expressed the gene in two tumor cell lines, H1299 and U2-OS. In both lines, forced AMPK-ß1 expression inhibits tumor cell growth, suggesting that AMPK-ß1 induction may facilitate stress-induced growth inhibition and cell killing.
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