Carcinogenesis Advance Access originally published online on May 13, 2004
Carcinogenesis 2004 25(10):1813-1820; doi:10.1093/carcin/bgh188
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Carcinogenesis vol.25 no.10 © Oxford University Press 2004; all rights reserved.
ARTICLE |
Sodium butyrate sensitizes TRAIL-mediated apoptosis by induction of transcription from the DR5 gene promoter through Sp1 sites in colon cancer cells
1 Department of Immunology, School of Medicine, Keimyung University, 194 DongSan-Dong Jung-Gu, Taegu, 700-712, South Korea and 2 Department of Microbiology, College of Natural Sciences, Kyungpook National University, Taegu, South Korea
3 To whom correspondence should be addressed Email: kwontk{at}dsmc.or.kr
Sodium butyrate, a short-chain fatty acid naturally present in the human colon, is able to induce cell cycle arrest, differentiation and apoptosis in various cancer cells. Sodium butyrate is most probably related to the inhibition of deacetylases leading to hyperacetylation of chromatin components such as histones and non-histone proteins and to alterations in gene expression. In this study, we demonstrate for the first time that sodium butyrate selectively up-regulated DR5 but had no effect on the expression of the other TNF-
-related apoptosis-inducing ligand (TRAIL) receptor, DR4. Sodium butyrate-induced expression of DR5 involves the putative Sp1 site within the DR5 promoter region. Using a combination of the electrophoretic mobility shift assay and the luciferase reporter assay, we found that a specific Sp1 site (located at –195 bp relative to the transcription start site) is required for sodium butyrate-mediated activation of the DR5 promoter. When HCT116 cells were incubated with sodium butyrate and TRAIL, enhanced TRAIL-mediated apoptosis was observed. The enhanced apoptosis was measured by fluorescent activated cell sorting analysis, DNA fragmentation, poly (ADP-ribose) polymerase cleavage, down-regulation of XIAP and caspase activity. Taken together, the present studies suggest that sodium butyrate may be an effective sensitizer of TRAIL-induced apoptosis.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  H Schulze-Bergkamen, A Weinmann, M Moehler, J Siebler, and P R Galle Novel ways to sensitise gastrointestinal cancer to apoptosis Gut, July 1, 2009; 58(7): 1010 - 1024. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Lim, J.-W. Park, K. S. Choi, Y. B. Park, and T. K. Kwon Rottlerin induces apoptosis via death receptor 5 (DR5) upregulation through CHOP-dependent and PKC {delta}-independent mechanism in human malignant tumor cells Carcinogenesis, May 1, 2009; 30(5): 729 - 736. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Yoshioka, H. Kamitani, T. Watanabe, and T. E. Eling Nonsteroidal Anti-inflammatory Drug-activated Gene (NAG-1/GDF15) Expression Is Increased by the Histone Deacetylase Inhibitor Trichostatin A J. Biol. Chem., November 28, 2008; 283(48): 33129 - 33137. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-J. Chen, C.-W. Chou, Y.-F. Chang, and C.-C. Chen Proteasome Inhibitors Enhance TRAIL-Induced Apoptosis through the Intronic Regulation of DR5: Involvement of NF-{kappa}B and Reactive Oxygen Species-Mediated p53 Activation J. Immunol., June 15, 2008; 180(12): 8030 - 8039. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. T. Nawrocki, J. S. Carew, L. Douglas, J. L. Cleveland, R. Humphreys, and J. A. Houghton Histone Deacetylase Inhibitors Enhance Lexatumumab-Induced Apoptosis via a p21Cip1-Dependent Decrease in Survivin Levels Cancer Res., July 15, 2007; 67(14): 6987 - 6994. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C.-Y. Jin, D.-O. Moon, J.-D. Lee, M.-S. Heo, Y. H. Choi, C.-M. Lee, Y.-M. Park, and G.-Y. Kim Sulforaphane sensitizes tumor necrosis factor-related apoptosis-inducing ligand-mediated apoptosis through downregulation of ERK and Akt in lung adenocarcinoma A549 cells Carcinogenesis, May 1, 2007; 28(5): 1058 - 1066. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Baritaki, S. Huerta-Yepez, T. Sakai, D. A. Spandidos, and B. Bonavida Chemotherapeutic drugs sensitize cancer cells to TRAIL-mediated apoptosis: up-regulation of DR5 and inhibition of Yin Yang 1 Mol. Cancer Ther., April 1, 2007; 6(4): 1387 - 1399. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. van Geelen, J. L. Westra, E. G. de Vries, W. Boersma-van Ek, N. Zwart, H. Hollema, H. M. Boezen, N. H. Mulder, J. T. Plukker, S. de Jong, et al. Prognostic Significance of Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand and Its Receptors in Adjuvantly Treated Stage III Colon Cancer Patients J. Clin. Oncol., November 1, 2006; 24(31): 4998 - 5004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Lei, M. Abdelrahim, and S. Safe 1,1-Bis(3'-indolyl)-1-(p-substituted phenyl)methanes inhibit ovarian cancer cell growth through peroxisome proliferator-activated receptor-dependent and independent pathways. Mol. Cancer Ther., September 1, 2006; 5(9): 2324 - 2336. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A Jungel, V Baresova, C Ospelt, B R Simmen, B A Michel, R E Gay, S Gay, C A Seemayer, and M Neidhart Trichostatin A sensitises rheumatoid arthritis synovial fibroblasts for TRAIL-induced apoptosis Ann Rheum Dis, July 1, 2006; 65(7): 910 - 912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. E. Joung, K. N. Min, J. Y. An, D.-K. Kim, G. Kong, and Y. Y. Sheen Potent In vivo Anti-Breast Cancer Activity of IN-2001, a Novel Inhibitor of Histone Deacetylase, in MMTV/c-Neu Mice. Cancer Res., May 15, 2006; 66(10): 5394 - 5402. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Imre, V. Gekeler, A. Leja, T. Beckers, and M. Boehm Histone Deacetylase Inhibitors Suppress the Inducibility of Nuclear Factor-{kappa}B by Tumor Necrosis Factor-{alpha} Receptor-1 Down-regulation. Cancer Res., May 15, 2006; 66(10): 5409 - 5418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Abdelrahim, K. Newman, K. Vanderlaag, I. Samudio, and S. Safe 3,3'-Diindolylmethane (DIM) and its derivatives induce apoptosis in pancreatic cancer cells through endoplasmic reticulum stress-dependent upregulation of DR5 Carcinogenesis, April 1, 2006; 27(4): 717 - 728. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kim, E. H. Kim, Y. W. Eom, W.-H. Kim, T. K. Kwon, S. J. Lee, and K. S. Choi Sulforaphane Sensitizes Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-Resistant Hepatoma Cells to TRAIL-Induced Apoptosis through Reactive Oxygen Species-Mediated Up-regulation of DR5 Cancer Res., February 1, 2006; 66(3): 1740 - 1750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. R. Rosato, J. A. Almenara, S. C. Maggio, P. Atadja, R. Craig, J. Vrana, P. Dent, and S. Grant Potentiation of the lethality of the histone deacetylase inhibitor LAQ824 by the cyclin-dependent kinase inhibitor roscovitine in human leukemia cells Mol. Cancer Ther., November 1, 2005; 4(11): 1772 - 1785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Jung, J. H. Lim, T. J. Lee, J.-W. Park, K. S. Choi, and T. K. Kwon Curcumin sensitizes tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-induced apoptosis through reactive oxygen species-mediated upregulation of death receptor 5 (DR5) Carcinogenesis, November 1, 2005; 26(11): 1905 - 1913. [Abstract] [Full Text] [PDF]
|
|