Skip Navigation


Carcinogenesis Advance Access originally published online on May 20, 2004
Carcinogenesis 2004 25(10):1839-1846; doi:10.1093/carcin/bgh190
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
25/10/1839    most recent
bgh190v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Baumann Kubetzko, F. B.
Right arrow Articles by Himmelmann, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baumann Kubetzko, F. B.
Right arrow Articles by Himmelmann, A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Carcinogenesis vol.25 no.10 © Oxford University Press 2004; all rights reserved.

ARTICLE

The PAX5 oncogene is expressed in N-type neuroblastoma cells and increases tumorigenicity of a S-type cell line

Florian B. Baumann Kubetzko1, Claudio di Paolo1, Charlotte Maag1, Roland Meier3, Beat W. Schäfer4, David R. Betts4, Rolf A. Stahel1 and Andreas Himmelmann1,2,5

1 Forschungslabor Molekulare Onkologie, Klinik und Poliklinik für Onkologie, Universitaetsspital Zurich, Haeldeliweg 4, CH-8044 Zurich, Switzerland, 2 Medizinische Klinik B, Universitaetsspital Zurich, Raemistrasse 100, CH-8091 Zurich, Switzerland, 3 Medizinische Klinik, Universitaets-Kinderklinik, Steinwiesstrasse 75, CH-8032 Zurich, Switzerland and 4 Abteilung für Onkologie, Universitaets-Kinderklinik, Steinwiesstrasse 75, CH-8032 Zurich, Switzerland

5 To whom correspondence should be addressed Email: andreas.himmelmann{at}usz.ch

Neuroblastoma is a neural crest-derived neoplasm of infancy with poor outcome in patients with advanced disease. The oncogenic transcription factor PAX5 is an important developmental regulator and is implicated in the pathogenesis of several malignancies. Screening of neuroblastoma cell lines revealed PAX5 expression in a malignant subset of neuroblastoma cells, so-called ‘N-type’ cells, but not in the more benign ‘S-type’ neuroblastoma cells. PAX5 expression was also detected in small cell lung cancer, an aggressive tumor of neural crest origin. Based on this observation we hypothesized that there could be a relationship between PAX5 expression and the more malignant phenotype of N-type cells. Stable PAX5 expression was established in several clones of the S-type cell line CA-2E. A noticeable difference in morphology of these transfectants was observed and there was also a significant increase in the proliferation rate. Moreover, PAX5 expressing clones gained the ability to form colonies in a soft agar assay, a marker of tumorigenicity. Down-regulation of PAX5 in several N-type cell lines and one small cell lung cancer cell line utilizing small interfering RNA resulted in a significant decrease in growth rate. Taken together we propose PAX5 as an important factor for the maintenance of the proliferative and tumorigenic phenotype of neuroblastoma. Our data, together with a recent study on the role of PAX genes in cancer suggest that PAX5 and other PAX transcription factors might be valuable targets for cancer therapy.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
M. Yan, N. Himoudi, M. Pule, N. Sebire, E. Poon, A. Blair, O. Williams, and J. Anderson
Development of Cellular Immune Responses against PAX5, a Novel Target for Cancer Immunotherapy
Cancer Res., October 1, 2008; 68(19): 8058 - 8065.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
G. A. Robichaud, J.-P. Perreault, and R. J. Ouellette
Development of an isoform-specific gene suppression system: the study of the human Pax-5B transcriptional element
Nucleic Acids Res., August 1, 2008; 36(14): 4609 - 4620.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
T. Brun and B. R Gauthier
A focus on the role of Pax4 in mature pancreatic islet {beta}-cell expansion and survival in health and disease
J. Mol. Endocrinol., February 1, 2008; 40(2): 37 - 45.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
P. Mhawech-Fauceglia, R. Saxena, S. Zhang, L. Terracciano, G. Sauter, A. Chadhuri, F. R Herrmann, and R. Penetrante
Pax-5 immunoexpression in various types of benign and malignant tumours: a high-throughput tissue microarray analysis
J. Clin. Pathol., June 1, 2007; 60(6): 709 - 714.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
A. Souabni, W. Jochum, and M. Busslinger
Oncogenic role of Pax5 in the T-lymphoid lineage upon ectopic expression from the immunoglobulin heavy-chain locus
Blood, January 1, 2007; 109(1): 281 - 289.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
T. Brun, I. Franklin, L. St-Onge, A. Biason-Lauber, E. J. Schoenle, C. B. Wollheim, and B. R. Gauthier
The diabetes-linked transcription factor PAX4 promotes {beta}-cell proliferation and survival in rat and human islets
J. Cell Biol., December 20, 2004; 167(6): 1123 - 1135.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.