Carcinogenesis Advance Access originally published online on November 21, 2003
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Carcinogenesis, Vol. 25, No. 3, 413-417,
March 2004
Carcinogenesis vol.25 no.3 © Oxford University Press 2004; all rights reserved.
CARCINOGENESIS |
Arsenic-induced DNA hypomethylation affects chromosomal instability in mammalian cells
Dipartimento di Biologia Cellulare e dello Sviluppo ‘A. Monroy’, Università di Palermo, Viale delle Scienze, Parco d'Orleans, I-90128 Palermo, Italy
Early genetic instability induced in dividing V79-Cl3 Chinese hamster cells by inorganic arsenic, as demonstrated in our previous investigation, was evidenced by aneuploidy and nuclear abnormalities, but not by chromosomal rearrangements. Here we report the results of cytogenetic and morphological analyses performed on the progeny of cells dividing at the end of sodium arsenite treatment after they had been expanded through 120 generations (ASO cells) and then cloned. The acquired genetic instability persisted and was increased by highly unstable chromosomal rearrangements, namely dicentric chromosomes and telomeric associations, which were not seen following acute exposure. A peculiar finding was the preferential involvement of a particular chromosome in dicentric rearrangements observed in some isolated ASO clones. Interestingly, by immunostaining with anti-5-methylcytosine antibodies the genome-wide DNA hypomethylation, induced by arsenic immediately after the acute treatment, was found to affect those ASO clones characterized by aneuploidy and chromosomal rearrangements. These findings demonstrate that short-term exposure to arsenic has long-term effects and suggest that genome-wide DNA hypomethylation enhances genetic instability.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. Liu and M. P. Waalkes Liver is a Target of Arsenic Carcinogenesis Toxicol. Sci., September 1, 2008; 105(1): 24 - 32. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.S. Young, B. Eskenazi, F.M. Marchetti, G. Block, and A.J. Wyrobek The association of folate, zinc and antioxidant intake with sperm aneuploidy in healthy non-smoking men Hum. Reprod., May 1, 2008; 23(5): 1014 - 1022. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. O. Wright and A. Baccarelli Metals and Neurotoxicology J. Nutr., December 1, 2007; 137(12): 2809 - 2813. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. V. Karpinets and B. D. Foy Tumorigenesis: the adaptation of mammalian cells to sustained stress environment by epigenetic alterations and succeeding matched mutations Carcinogenesis, August 1, 2005; 26(8): 1323 - 1334. [Abstract] [Full Text] [PDF]
|
|