Skip Navigation


Carcinogenesis Advance Access originally published online on February 19, 2004
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
25/7/1219    most recent
bgh117v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (25)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Lüchtenborg, M.
Right arrow Articles by de Goeij, A. F. P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lüchtenborg, M.
Right arrow Articles by de Goeij, A. F. P. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Carcinogenesis, Vol. 25, No. 7, 1219-1226, July 2004
Carcinogenesis vol.25 no.7 © Oxford University Press 2004; all rights reserved.

ARTICLE

APC mutations in sporadic colorectal carcinomas from The Netherlands Cohort Study

Margreet Lüchtenborg1,5, Matty P. Weijenberg1, Guido M. J. M. Roemen2, Adriaan P. de Bruïne3, Piet A. van den Brandt1, Marjolein H. F. M. Lentjes2, Mirian Brink1, Manon van Engeland3, R. Alexandra Goldbohm4 and Anton F. P. M. de Goeij3

1 Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Department of Epidemiology, 2 Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Department of Pathology and 3 Research Institute Growth and Development (GROW), Department of Pathology, University Maastricht, 6200 MD Maastricht and 4 TNO Nutrition and Food Research, 3700 AJ Zeist, The Netherlands

5 To whom correspondence should be addressed Email: m.luchtenborg{at}epid.unimaas.nl

The adenomatous polyposis coli (APC) gene is considered to be a gatekeeper in colorectal tumourigenesis. Inactivating mutations in APC have been reported in 34–70% of sporadic colorectal cancer patients, the majority of which occur in the mutation cluster region (MCR). In this study, tumour tissue from 665 incident colorectal cancer patients, who originate from 120 852 men and women (55–69 years of age at baseline) participating in The Netherlands Cohort Study, was evaluated for the occurrence and type of APC mutations with regard to age at diagnosis, gender, family history of colorectal cancer, Dukes' stage, tumour differentiation and sub-localization. Mutation analysis of the MCR, which spans codons 1286–1513, was performed on archival adenocarcinoma samples using macrodissection, nested PCR and direct sequencing of purified PCR fragments. A large number of genetic aberrations (n = 978), including point mutations (n = 833), deletions (n = 126) and insertions (n = 19) was detected in the MCR in 72% of patients (479/665). In particular, we observed a large number of missense mutations, more than reported previously. This may indicate involvement in colorectal carcinogenesis, although their significance for APC functions is unclear. Truncating mutations were found in 37% of patients (248/665). Patients with rectosigmoid and rectum tumours relatively more frequently harboured C > T nonsense mutations and truncating frameshift mutations as compared with patients with proximal and distal colon tumours (P = 0.009 and P = 0.045, respectively). Differences in occurrence of truncating mutations with regard to tumour sub-localization suggest a different aetiology of tumourigenesis in colon and rectum.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 CarcinogenesisHome page
M. Li, H. Edamatsu, R. Kitazawa, S. Kitazawa, and T. Kataoka
Phospholipase C{varepsilon} promotes intestinal tumorigenesis of ApcMin/+ mice through augmentation of inflammation and angiogenesis
Carcinogenesis, August 1, 2009; 30(8): 1424 - 1432.
[Abstract] [Full Text] [PDF]

Home page
 Ann OncolHome page
S. de Vogel, M. P. Weijenberg, J. G. Herman, K. A. D. Wouters, A. F. P. M. de Goeij, P. A. van den Brandt, A. P. de Bruine, and M. van Engeland
MGMT and MLH1 promoter methylation versus APC, KRAS and BRAF gene mutations in colorectal cancer: indications for distinct pathways and sequence of events
Ann. Onc., July 1, 2009; 20(7): 1216 - 1222.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
M. Mutoh, N. Niho, M. Komiya, M. Takahashi, R. Ohtsubo, K. Nakatogawa, K. Ueda, T. Sugimura, and K. Wakabayashi
Plasminogen activator inhibitor-1 (Pai-1) blockers suppress intestinal polyp formation in Min mice
Carcinogenesis, April 1, 2008; 29(4): 824 - 829.
[Abstract] [Full Text] [PDF]

Home page
 Am J EpidemiolHome page
A. Kuijsten, P. C. H. Hollman, H. C. Boshuizen, M. N. C. P. Buijsman, P. van 't Veer, F. J. Kok, I. C. W. Arts, and H. B. Bueno-de-Mesquita
Plasma Enterolignan Concentrations and Colorectal Cancer Risk in a Nested Case-Control Study
Am. J. Epidemiol., March 15, 2008; 167(6): 734 - 742.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
E. H. Lips, R. van Eijk, E. J.R. de Graaf, P. G. Doornebosch, N. F.C.C. de Miranda, J. Oosting, T. Karsten, P. H.C. Eilers, R. A.E.M. Tollenaar, T. van Wezel, et al.
Progression and Tumor Heterogeneity Analysis in Early Rectal Cancer
Clin. Cancer Res., February 1, 2008; 14(3): 772 - 781.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
A. Sanchez-de-Abajo, M. de la Hoya, M. van Puijenbroek, A. Tosar, J.A. Lopez-Asenjo, E. Diaz-Rubio, H. Morreau, and T. Caldes
Molecular Analysis of Colorectal Cancer Tumors from Patients with Mismatch Repair Proficient Hereditary Nonpolyposis Colorectal Cancer Suggests Novel Carcinogenic Pathways
Clin. Cancer Res., October 1, 2007; 13(19): 5729 - 5735.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
A. P. Femia, P. Dolara, A. Giannini, M. Salvadori, A. Biggeri, and G. Caderni
Frequent Mutation of Apc Gene in Rat Colon Tumors and Mucin-Depleted Foci, Preneoplastic Lesions in Experimental Colon Carcinogenesis
Cancer Res., January 15, 2007; 67(2): 445 - 449.
[Abstract] [Full Text] [PDF]

Home page
 J. Nutr.Home page
S. de Vogel, M. van Engeland, M. Luchtenborg, A. P. de Bruine, G. M. J. M. Roemen, M. H. F. M. Lentjes, R. A. Goldbohm, P. A. van den Brandt, A. F. P. M. de Goeij, and M. P. Weijenberg
Dietary Folate and APC Mutations in Sporadic Colorectal Cancer
J. Nutr., December 1, 2006; 136(12): 3015 - 3021.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
M Nielsen, J W Poley, S Verhoef, M van Puijenbroek, M M Weiss, G T Burger, C J Dommering, H F A Vasen, E J Kuipers, A Wagner, et al.
Duodenal carcinoma in MUTYH-associated polyposis
J. Clin. Pathol., November 1, 2006; 59(11): 1212 - 1215.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
G R van den Brink and J C H Hardwick
Hedgehog Wnteraction in colorectal cancer
Gut, July 1, 2006; 55(7): 912 - 914.
[Full Text] [PDF]

Home page
 J. Mol. Diagn.Home page
T. Sugai, W. Habano, Y.-F. Jiao, M. Tsukahara, Y. Takeda, K. Otsuka, and S.-i. Nakamura
Analysis of Molecular Alterations in Left- and Right-Sided Colorectal Carcinomas Reveals Distinct Pathways of Carcinogenesis: Proposal for New Molecular Profile of Colorectal Carcinomas
J. Mol. Diagn., May 1, 2006; 8(2): 193 - 201.
[Abstract] [Full Text] [PDF]

Home page
 Am J EpidemiolHome page
M. Luchtenborg, M. P. Weijenberg, E. Kampman, G. N. van Muijen, G. M. J. M. Roemen, M. P. A. Zeegers, R. A. Goldbohm, P. van 't Veer, A. F. P. M. de Goeij, and P. A. van den Brandt
Cigarette Smoking and Colorectal Cancer: APC Mutations, hMLH1 Expression, and GSTM1 and GSTT1 Polymorphisms
Am. J. Epidemiol., May 1, 2005; 161(9): 806 - 815.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.