Skip Navigation


Carcinogenesis Advance Access originally published online on September 16, 2004
Carcinogenesis 2005 26(1):37-43; doi:10.1093/carcin/bgh280
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Supplementary Data
Right arrow All Versions of this Article:
26/1/37    most recent
bgh280v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (14)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Chen, J.
Right arrow Articles by Ebert, M. P.A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, J.
Right arrow Articles by Ebert, M. P.A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Carcinogenesis vol.26 no.1 © Oxford University Press 2005; all rights reserved.

ARTICLE

Molecular analysis of APC promoter methylation and protein expression in colorectal cancer metastasis

Jie Chen1, Christoph Röcken2, Cathy Lofton-Day4, Hans-Ulrich Schulz3, Oliver Müller5, Nadine Kutzner5, Peter Malfertheiner1 and Matthias P.A. Ebert1,6

1 Department of Gastroenterology, Hepatology and Infectious Diseases, 2 Institute of Pathology and 3 Department of General Surgery, Otto-von-Guericke University, Magdeburg, Germany, 4 Epigenomics Inc., Seattle, Washington, USA and 5 Max-Planck-Institute of Molecular Physiology, Dortmund, Germany

6 To whom correspondence should be addressed Email: matthias.ebert{at}medizin.uni-magdeburg.de

APC (adenomatous polyposis coli) promoter methylation has been linked to the early development of colorectal cancers. However, the role of APC methylation and its effect on protein expression in colon cancer metastasis is largely unknown. In this study, we investigated APC promoter methylation by Methylight analysis and analysed the APC protein levels by immunohistochemistry and western blot analysis in 24 liver metastasis and 39 primary colorectal cancers. Promoter methylation of the APC gene was found to be a frequent event in liver metastasis (10/24) and significantly more frequent compared with primary colorectal cancer (7/39, P = 0.047). APC methylation was not found in 14 matched normal colon tissues. APC protein was detected in the cytoplasm of primary and metastatic cancer cells and non-tumorous colon epithelium. By western blot analysis, APC protein levels were found to be decreased in primary tumour tissues compared with the normal colon mucosa. In contrast, APC protein levels were not decreased in the cancer cells that had metastasized to the liver. APC protein levels were independent of the presence of APC promoter methylation or gene mutations. In summary, APC promoter methylation is a frequent epigenetic alteration in colorectal cancer metastasis. However, we observed no significant association between APC promoter methylation or gene mutation and APC protein expression in colorectal metastasis. Therefore, metastatic cancer cells seem to harbour a heterogenous genetic and epigenetic background, in which cancer cells may exhibit APC promoter methylation that is independent of APC expression.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Cancer ResHome page
J. M. McGough, D. Yang, S. Huang, D. Georgi, S. M. Hewitt, C. Rocken, M. Tanzer, M. P.A. Ebert, and K. Liu
DNA Methylation Represses IFN-{gamma}-Induced and Signal Transducer and Activator of Transcription 1-Mediated IFN Regulatory Factor 8 Activation in Colon Carcinoma Cells
Mol. Cancer Res., December 1, 2008; 6(12): 1841 - 1851.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
A. A. Baran, K. A. Silverman, J. Zeskand, R. Koratkar, A. Palmer, K. McCullen, W. J. Curran Jr, T. B. Edmonston, L. D. Siracusa, and A. M. Buchberg
The modifier of Min 2 (Mom2) locus: Embryonic lethality of a mutation in the Atp5a1 gene suggests a novel mechanism of polyp suppression
Genome Res., May 1, 2007; 17(5): 566 - 576.
[Abstract] [Full Text] [PDF]

Home page
 GutHome page
M Hitchins, C Suter, J Wong, K Cheong, N Hawkins, B Leggett, R Scott, A Spigelman, I Tomlinson, D Martin, et al.
Germline epimutations of APC are not associated with inherited colorectal polyposis.
Gut, April 1, 2006; 55(4): 586 - 587.
[Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
P. Schatz, J. Distler, K. Berlin, and M. Schuster
Novel method for high throughput DNA methylation marker evaluation using PNA-probe library hybridization and MALDI-TOF detection.
Nucleic Acids Res., January 1, 2006; 34(8): e59 - e59.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
V. Schmiemann, A. Bocking, M. Kazimirek, A. S. C. Onofre, H. E. Gabbert, R. Kappes, C. D. Gerharz, and H. J. Grote
Methylation Assay for the Diagnosis of Lung Cancer on Bronchial Aspirates: A Cohort Study
Clin. Cancer Res., November 1, 2005; 11(21): 7728 - 7734.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.