Carcinogenesis Advance Access originally published online on December 9, 2004
Carcinogenesis 2005 26(3):613-620; doi:10.1093/carcin/bgh353
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Carcinogenesis vol.26 no.3 © Oxford University Press 2004; all rights reserved.
ARTICLE |
Ultrafine particulate matter and high-level benzene urban air pollution in relation to oxidative DNA damage
1 Laboratoire de Biochimie et de Biologie Moléculaire, Faculté des Sciences et Techniques, Université d'Abomey-Calavi, Republique du Benin, 2 Institute of Public Health, University of Copenhagen, Denmark, 3 Department of Environmental and Occupational Medicine, Aarhus University, Denmark and 4 Unité d'enseignement et de recherché au travail et environnment, Faculté des Sciences de la Santé, Université d'Abomey-Calavi, Republique du Benin
5 To whom correspondence should be addressed Email: p.moller{at}pubhealth.ku.dk
Air pollution, containing high-level of ultrafine particles (UFP) and benzene, is a prominent environmental health problem in many cities of the World. We investigated the level of oxidative DNA damage in mononuclear blood cells (MNBC) by the comet assay as DNA strand breaks (SB) and formamidopyrimidine DNA glycosylase (FPG) sensitive sites in residents from three urban locations in Cotonou, Benin (taxi-moto drivers, subjects living near roads with intense traffic and suburban residents) and rural residents. Exposure was characterized by urinary excretion of S-phenylmercapturic acid (S-PMA), a biomarker of benzene exposure, and by ambient UFP. There were clear stepwise gradients with respect to ambient UFP, S-PMA excretion and oxidative DNA damage with rural subjects < suburban subjects < residents living near highly trafficed roads<taxi-moto drivers. Polymorphisms in glutathione peroxidase (GPX), NAD(P)H:quinone oxidoreductase 1 (NQO1) and glutathione S-transferase (GST) genes were assessed for effect modification. Subjects with GSTT1 null genotype had lower urinary S-PMA excretion than subjects carrying the plus genotype. Urinary S-PMA excretion correlated with SB (R = 0.17) and FPG sites (R = 0.25) in MNBC. The correlation between S-PMA and SB was strongest in subjects with NQO1*1/*2 and *2/*2 genotypes (R = 0.37), and between S-PMA and FPG sensitive sites in subjects with the GSTP1*B/*B genotype (R = 0.39). In conclusion, this study shows that urban air with high levels of benzene and UFP is associated with elevated levels of SB and FPG sites in MNBC, and that NQO1 and GST genes may modulate the effect.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  L.-C. Lin, W.-J. Chen, Y.-M. Chiung, T.-S. Shih, and P.-C. Liao Association between GST Genetic Polymorphism and Dose-Related Production of Urinary Benzene Metabolite Markers, trans, trans-Muconic Acid and S-Phenylmercapturic Acid Cancer Epidemiol. Biomarkers Prev., June 1, 2008; 17(6): 1460 - 1469. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. D. Houle, T.-V. T. Ton, N. Clayton, J. Huff, H.-H. L. Hong, and R. C. Sills Frequent p53 and H-ras Mutations in Benzene- and Ethylene Oxide-Induced Mammary Gland Carcinomas from B6C3F1 Mice Toxicol Pathol, October 1, 2006; 34(6): 752 - 762. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P E Schwarze, J Ovrevik, M Lag, M Refsnes, P Nafstad, R B Hetland, and E Dybing Particulate matter properties and health effects: consistency of epidemiological and toxicological studies Human and Experimental Toxicology, October 1, 2006; 25(10): 559 - 579. [Abstract] [PDF]
|
|
|
|
 |
|
 M. A. A. Schoonen, C. A. Cohn, E. Roemer, R. Laffers, S. R. Simon, and T. O'Riordan Mineral-Induced Formation of Reactive Oxygen Species Reviews in Mineralogy and Geochemistry, January 1, 2006; 64(1): 179 - 221. [Full Text] [PDF]
|
|