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Carcinogenesis Advance Access originally published online on April 28, 2005
Carcinogenesis 2005 26(9):1513-1519; doi:10.1093/carcin/bgi106
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Carcinogenesis vol.26 no.9 © Oxford University Press 2005; all rights reserved.

The initiation of colon cancer in a chronic inflammatory setting

Ru Chen, Peter S. Rabinovitch 1, David A. Crispin, Mary J. Emond 2, Mary P. Bronner 3, {dagger} and Teresa A. Brentnall *, {dagger}

Department of Medicine, 1 Department of Pathology and 2 Department of Biostatistics, University of Washington, Seattle, WA 98195, USA and 3 Department of Anatomic Pathology, Cleveland Clinic Foundation Cleveland, OH 44195, USA

* To whom correspondence should be addressed at: Department of Medicine, Box 356424, University of Washington, Seattle, WA 98195, USA. Tel: +1 206 543 3280; Fax: +1 206 685 9478; Email: teribr{at}u.washington.edu

Chronic inflammation predisposes to cancer. We used an inflammation-induced human model of tumorigenesis to explore how populations of mutated cells expand and initiate the earliest stages of cancer. Ulcerative colitis (UC) is a chronic inflammatory disease of the colon associated with an increased risk of colorectal cancer mediated through a process of genomic instability. In order to characterize the process of clonal expansion, arbitrary primed (AR) and inter-simple sequence repeat (ISSR) PCR DNA fingerprint mutation profiles of single crypts were compared with the mutational profiles from clusters of crypts and whole biopsies within the same individual. To provide information at the earliest steps of neoplastic progression, we examined histologically negative crypts, as well as dysplastic crypts. Crypts from UC dysplasia/cancer show alterations in 10–20% of DNA fingerprint sites, regardless of (i) whether the crypts were dysplastic or non-dysplastic and (ii) whether the DNA came from one crypt or thousands of crypts. Of the mutational changes in single crypts, almost half are clonally expanded to adjacent crypts and/or to the thousands of crypts in a single biopsy. Using fluorescent in-situ hybridization to examine p53 alterations in individual crypt cells, we demonstrate that the mechanism of clonal expansion can occur through crypt fission. DNA alterations are initiated in colonic crypts and expand to adjacent crypts through crypt fission. Our data suggest that a continuous process of DNA mutations, clonal expansion through crypt fission and clonal succession initiates the development of inflammatory-associated colon cancer; this mutational process is moderated by crypt cell turn-over and cell death. This paradigm may apply to other inflammatory-induced cancers.


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