Skip Navigation


Carcinogenesis Advance Access originally published online on December 19, 2005
Carcinogenesis 2006 27(5):936-944; doi:10.1093/carcin/bgi316
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrowOA All Versions of this Article:
27/5/936    most recent
bgi316v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Google Scholar
Right arrow Articles by Hsiao, H.-L.
Right arrow Articles by Su, Y.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hsiao, H.-L.
Right arrow Articles by Su, Y.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2005. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Overexpression of thymosin ß-4 renders SW480 colon carcinoma cells more resistant to apoptosis triggered by FasL and two topoisomerase II inhibitors via downregulating Fas and upregulating Survivin expression, respectively

Hung-Liang Hsiao, Wei-Shu Wang 1, Po-Min Chen 1 and Yeu Su 2, *

Institute of Pharmacology, College of Medicine, National Yang-Ming University, Shih-Pai, Taipei 11221, Taiwan, R.O.China, 1 Division of Medical Oncology, Department of Medicine, Taipei Veterans General Hospital, Taipei, Taiwan, R.O.China and 2 Institute of Biopharmaceutical Science, Collage of Life Science, National Yang-Ming University, Taipei, Taiwan, R.O.China

* To whom correspondence should be addressed. Email: yeusu{at}ym.edu.tw

The present work was conducted to further examine the effects of thymosin ß-4 (Tß4) upregulation on the apoptosis of SW480 colon cancer cells induced by T cells and various chemotherapeutic agents because reduced susceptibility to the cytotoxicity of an anti-Fas IgM (CH-11) in Tß4-overexpressing cells has previously been reported by us. As expected, Tß4 overexpressers were also more resistant to the killing effect of FasL-bearing Jurkat T cells. On the other hand, pretreating these cells with an MMP inhibitor restored not only their Fas levels but also their sensitivity to CH-11, suggesting a pivotal role of MMP in downregulating Fas in Tß4 overexpressers. Interestingly, while the susceptibilities of Tß4 overexpressers to 5-FU and irinotecan remained unchanged, they were more resistant to doxorubicin and etoposide which triggered apoptosis via a mitochondrial pathway. Concordantly, activation of both caspases 9 and 3 in Tß4 overexpressers by the two aforementioned topoisomerase II inhibitors was dramatically abrogated which could be accounted mainly by an increased expression of Survivin, a critical anti-apoptotic factor. Finally, poor survival was found in stage III colon cancer patients whose tumors were stained positively by the anti-Survivin antibody. Thus, advantages such as immune evasion and resistance to anticancer drug-induced apoptosis acquired by colon cancer cells through Tß4 overexpression might facilitate their survival during metastasis and chemotherapy.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J AndrolHome page
K. Iguchi, M. Ito, S. Usui, A. Mizokami, M. Namiki, and K. Hirano
Downregulation of Thymosin {beta}4 Expression by Androgen in Prostate Cancer LNCaP Cells
J Androl, March 1, 2008; 29(2): 207 - 212.
[Abstract] [Full Text] [PDF]

Home page
 IOVSHome page
J. H.-C. Ho, C.-H. Chuang, C.-Y. Ho, Y.-R. V. Shih, O. K.-S. Lee, and Y. Su
Internalization Is Essential for the Antiapoptotic Effects of Exogenous Thymosin {beta}-4 on Human Corneal Epithelial Cells
Invest. Ophthalmol. Vis. Sci., January 1, 2007; 48(1): 27 - 33.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.