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Carcinogenesis Advance Access originally published online on December 12, 2005
Carcinogenesis 2006 27(6):1222-1231; doi:10.1093/carcin/bgi306
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© The Author 2005. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

A proteomic investigation into a human gastric cancer cell line BGC823 treated with diallyl trisulfide

Na Li 1, 2, 4, {dagger}, Ruifang Guo 3, {dagger}, Wenmei Li 3,, Jianmin Shao 1, 2, Shuting Li 1, 2, Kang Zhao 1, 2, Xishu Chen 1, 2, Ningzhi Xu 1, 2, Siqi Liu 1, 2 and Youyong Lu 1, 2, 3, *

1 Beijing Genomics Institute, Chinese Academy of Sciences and 2 Beijing Proteomics Institute, Beijing Airport Industrial Zone B-6, Shunyi, Beijing 101300, China, 3 Peking University School of Oncology, Beijing Institute for Cancer Research, Beijing 100034, China and 4 Graduate School of Chinese Academy of Sciences, Beijing 100049, China

* To whom correspondence should be addressed. Tel: +86 10 6616 3061; Fax: +86 10 6617 5832; Email: yongylu{at}public.bta.net.cn

Garlic is generally used as a therapeutic reagent against various diseases worldwide. Although a great effort is made to understand the pharmaceutical mechanisms of garlic and its derivatives, there are many mysteries to be uncovered. Using proteomic means, herein we have systematically studied the responses of protein expression in BGC823 cells, a gastric cancer cell line, induced by diallyl trisulfide (DATS), a major component of garlic derivatives. A total of 41 unique proteins in BGC823 were detected with significant changes in their expression levels corresponding with DATS administration. Of these proteins, five typical ones, glutathione S-transferase-pi (GST-pi), voltage-dependent anion channel-1 (VDAC-1), Annexin I, Galectin and S100A11, were further examined by Western blotting, resulting in coincident data with the proteomic evidence. Moreover quantitative real-time RT–PCR experiments offered dynamic data of mRNA expression, indicating the responses of Annexin I and GST-pi expression within a short period after DATS treatment. Interestingly, ~50% of DATS-sensitive proteins (19/41) in BGC823 are tightly associated with apoptotic pathways. These proteomic results presented, therefore, provide additional support to the hypothesis that garlic is a strong inducer of apoptosis in tumor cells.


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