Carcinogenesis Advance Access originally published online on January 9, 2006
Carcinogenesis 2006 27(7):1398-1403; doi:10.1093/carcin/bgi337
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Effect of short-term fasting on urinary excretion of primary lipid peroxidation products and on markers of oxidative DNA damage in healthy women
1 Department of Preventive Medicine, Seoul National University College of Medicine, Institute for Environmental Medicine, SNUMRC, Seoul 110-799, Korea, 2 German Cancer Research Center (DKFZ), Division of Toxicology and Cancer Risk Factors, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
* To whom correspondence should be addressed at: Department of Preventive Medicine, Seoul National University College of Medicine, Institute of Environmental Medicine, SNUMRC, 28 Yongon-Dong Chongno-Gu, Seoul 110-799, Korea. Tel: +82 2 740 8326; Fax: +82 2 747 4830; E-mail: dhkang{at}snu.ac.kr
The goal of this study was to determine whether short-term fasting changes in urinary biomarkers related to oxidative stress: malondialdehyde (MDA), 8-isoprostaglandin F2
(8-isoPGF), 8-hydroxydeoxy-guanosine (8-OHdG) and 1,N6-ethenodeoxyadenosine (
dA) among female volunteers participating in the short-term fasting program in South Korea. The study subjects were 52 healthy women (mean age 28, range 15–48 years old) who provided urine samples both before and after the fasting program (average 7.2, range: 3–11 days). Urinary MDA was measured by HPLC-UV and dA levels were measured by immuno-affinity purification followed by HPLC-fluorescence detection. Urinary 8-OHdG and 8-isoPGF concentrations were determined by ELISA. Plasma leptin levels were also measured by radioimmunoassay. Information on demographic characteristics, personal habits (smoking and alcohol consumption) and previous medical history were collected by a self-administered questionnaire. Percent loss of body weight (average 6.3%, 4.28 ± 0.25 kg) was significantly correlated with fasting duration (r = 0.70, n = 52, P < 0.01). The plasma leptin levels after fasting (5.89 ± 1.10 ng/ml) were significantly lower than before fasting (6.91 ± 1.13 ng/ml) (n = 27, P = 0.05). Urinary MDA levels after fasting (0.18 ± 1.10 mg/g creatinine) were significantly lower than before fasting (0.37 ± 1.11) (n = 51, P < 0.01). Urinary 8-isoPGF also were significantly reduced after fasting (n = 47, P < 0.01). However, there was no significant difference in 8-OHdG or dA. There was a statistically significant correlation between % change of urinary MDA level with % change of 8-isoPGF level (partial correlation coefficient r = 0.57, n = 46, P = 0.01). The correlations between % change of 8-OHdG and plasma leptin was also significant (partial correlation coefficient r = 0.51, n = 27, P = 0.02). Our results demonstrate that the short-term fasting reduces lipid peroxidation products but does not affect oxidative stress-induced DNA damage.
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