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Carcinogenesis Advance Access originally published online on December 12, 2005
Carcinogenesis 2006 27(8):1526-1537; doi:10.1093/carcin/bgi311
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© The Author 2005. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Investigating human cancer etiology by DNA lesion footprinting and mutagenicity analysis

Ahmad Besaratinia* and Gerd P. Pfeifer

Division of Biology, Beckman Research Institute of the City of Hope National Medical Center 1450 East Duarte Road, Duarte, CA 91010, USA

*To whom correspondence should be addressed. Tel: +1 626 256 4673 ext. 65918; Fax: +1 626 358 7703; Email: ania{at}coh.org

Many genotoxic carcinogens are known to leave unique signatures on cancer-related genes. The signature of carcinogens is manifested by the induction of characteristic mutations at distinctive nucleotide positions along oncogenes and/or tumor suppressor genes. Often, the nucleotide positions, wherein mutations occur, co-localize with the sites of initial DNA damage induced by the respective carcinogens. Thus, DNA damage-targeted mutation can be a predictor of carcinogenicity of genotoxins. Today, genomic sequencing technologies for investigating human cancer etiology are based on DNA-lesion footprinting in conjunction with mutagenicity analysis of genotoxic carcinogens. In this review article, we discuss the ligation-mediated PCR and terminal transferase-dependent-PCR, two versatile DNA-lesion footprinting techniques. We highlight the in vitro shuttle vector-based mutation systems for investigating site-specific mutagenicity of carcinogens and the in vivo transgenic rodent mutation systems for exploring DNA damaging and mutagenic properties of carcinogens. We present examples of application of each of these methodologies to human cancer etiology, and provide prospective views on investigations using these technologies for carcinogenicity testing.


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