Skip Navigation


Carcinogenesis Advance Access originally published online on February 10, 2006
Carcinogenesis 2006 27(8):1567-1578; doi:10.1093/carcin/bgi339
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow All Versions of this Article:
27/8/1567    most recent
bgi339v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (24)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Buterin, T.
Right arrow Articles by Naegeli, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Buterin, T.
Right arrow Articles by Naegeli, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© The Author 2006. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

Convergent transcriptional profiles induced by endogenous estrogen and distinct xenoestrogens in breast cancer cells

Tonko Buterin, Caroline Koch and Hanspeter Naegeli*

Institute of Pharmacology and Toxicology, University of Zürich-Vetsuisse Winterthurerstrasse 260, 8057 Zürich, Switzerland

*To whom correspondence should be addressed. Tel: +41 1 635 87 63; Fax: +41 1 635 89 10; Email: naegelih{at}vetpharm.unizh.ch

Estrogen receptors display high levels of promiscuity in accommodating a wide range of ligand structures, but the functional consequence of changing receptor conformations in complex with distinct agonists is highly controversial. To determine variations in the transactivation capacity induced by different estrogenic agonists, we assessed global transcriptional profiles elicited by natural or synthetic xenoestrogens in comparison with the endogenous hormone 17ß-estradiol. Human MCF7 and T47D carcinoma cells, representing the most frequently used model systems for tumorigenic responses in the mammary gland, were synchronized by hormone starvation during 48 h. Subsequently, a 24 h exposure was carried out with equipotent concentrations of the selected xenoestrogens or 17ß-estradiol. Analysis of messenger RNA was performed on high-density oligonucleotide microarrays that display the sequences of 33 000 human transcripts, yielding a total of 181 gene products that are regulated upon estrogenic stimulation. Surprisingly, genistein (a phytoestrogen), bisphenol-A and polychlorinated biphenyl congener 54 (two synthetic xenoestrogens) produced highly congruent genomic fingerprints by regulating the same range of human genes. Also, the monotonous genomic signature observed in response to xenoestrogens is identical to the transcriptional effects induced by physiological concentrations of 17ß-estradiol. This striking functional convergence indicates that the transcription machinery is largely insensitive to the particular structure of estrogen receptor agonists. The occurrence of such converging transcriptional programs reinforces the hypothesis that multiple xenoestrogenic contaminants, of natural or anthropogenic origin, may act in conjunction with the endogenous hormone to induce additive effects in target tissues.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Cancer ResHome page
P. Singh, M. Yang, H. Dai, D. Yu, Q. Huang, W. Tan, K. H. Kernstine, D. Lin, and B. Shen
Overexpression and Hypomethylation of Flap Endonuclease 1 Gene in Breast and Other Cancers
Mol. Cancer Res., November 1, 2008; 6(11): 1710 - 1717.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
A. M. Sotoca Covaleda, H. van den Berg, J. Vervoort, P. van der Saag, A. Strom, J.-A. Gustafsson, I. Rietjens, and A. J. Murk
Influence of Cellular ER{alpha}/ER{beta} Ratio on the ER{alpha}-Agonist Induced Proliferation of Human T47D Breast Cancer Cells
Toxicol. Sci., October 1, 2008; 105(2): 303 - 311.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
S. H. Dairkee, J. Seok, S. Champion, A. Sayeed, M. Mindrinos, W. Xiao, R. W. Davis, and W. H. Goodson
Bisphenol A Induces a Profile of Tumor Aggressiveness in High-Risk Cells from Breast Cancer Patients
Cancer Res., April 1, 2008; 68(7): 2076 - 2080.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
R. Dip, S. Lenz, J.-P. Antignac, B. Le Bizec, H. Gmuender, and H. Naegeli
Global gene expression profiles induced by phytoestrogens in human breast cancer cells
Endocr. Relat. Cancer, March 1, 2008; 15(1): 161 - 173.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
R. Moral, R. Wang, I. H Russo, C. A Lamartiniere, J. Pereira, and J. Russo
Effect of prenatal exposure to the endocrine disruptor bisphenol A on mammary gland morphology and gene expression signature
J. Endocrinol., January 1, 2008; 196(1): 101 - 112.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
D. Gallo, E. Mantuano, M. Fabrizi, C. Ferlini, S. Mozzetti, I. De Stefano, and G. Scambia
Effects of a phytoestrogen-containing soy extract on the growth-inhibitory activity of ICI 182 780 in an experimental model of estrogen-dependent breast cancer
Endocr. Relat. Cancer, June 1, 2007; 14(2): 317 - 324.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.