Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Appel, K. E.
Right arrow Articles by Graf, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Appel, K. E.
Right arrow Articles by Graf, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1982 Oxford University Press

research-article

Metabolic nitrite formation from N-nitrosamines: evidence for a cytochrome P-450 dependent reaction

Klaus E. Appel 1 and Hermann Graf 2

1Section of Toxicology, Institute of Pharmacology Obere Zahlbacher Str. 67, D-6500 Mainz
2Institute of Physiological Chemistry, University of the Saarland D-6650 Homburg, FRG

Nitrite was formed on incubation of N-nitrosamines with a reconstituted monooxygenase system, consisting of cytochrome P-450 (P-450) and NADPH P-450 reductase from pig liver. Nitrite was not obtained when the nitrosamines were incubated with NADPH P-450 reductase alone or when molecular oxygen or NADPH were omitted. Interaction of nitrosamines with the reconstituted P-450 system or with hemoglobin under reducing conditions resulted in optical spectra identical with those obtained with nitrite. It is proposed that N-nitrosamines are denitrosated by electron transfer from the hemoprotein iron to the nitrosamine molecule.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 CarcinogenesisHome page
J. F. Teiber and P. F. Hollenberg
Identification of the human liver microsomal cytochrome P450s involved in the metabolism of N-nitrosodi-n-propylamine
Carcinogenesis, August 1, 2000; 21(8): 1559 - 1566.
[Abstract] [Full Text] [PDF]

Home page
 CirculationHome page
A. Mulsch, P. Mordvintcev, E. Bassenge, F. Jung, B. Clement, and R. Busse
In Vivo Spin Trapping of Glyceryl Trinitrate–Derived Nitric Oxide in Rabbit Blood Vessels and Organs
Circulation, October 1, 1995; 92(7): 1876 - 1882.
[Abstract] [Full Text]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.