Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Barrows, L. R.
Right arrow Articles by Magee, P. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barrows, L. R.
Right arrow Articles by Magee, P. N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1982 Oxford University Press

other

Nonenzymatic methylation of DNA by S-adenosylmethionine in vitro

Louis R. Barrows 1 and Peter N. Magee 1 2

1Fels Research Institute, Temple University, School of Medicine Philadelphia, PA 19140, USA
2Department of Pathology, Temple University, School of Medicine Philadelphia, PA 19140, USA

S-Adenosylmethionine was found to methylate DNA non-enzymatically to produce the same putative promutagenic and procarcinogenic lesions formed by carcinogenic chemical methylating agents. The formation of 7-methylguanine was confirmed by u.v. spectrophotometry, the formation of O6-methylguanine and 3-methyladenine was suggested by the cochromatography of radioactivity with standard bases. It is possible that this reaction may explain the presence of constitutive cellular enzymes specifically for the repair of methylated DNA, and may indicate the mechanism whereby methylated guanine is formed in the liver DNA of rats with chemically induced liver damage.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
A. Tomita-Mitchell, L. L. Ling, C. L. Glover, J. Goodluck-Griffith, and W. G. Thilly
The Mutational Spectrum of the HPRT Gene from Human T Cells in Vivo Shares a Significant Concordant Set of Hot Spots with MNNG-treated Human Cells
Cancer Res., September 15, 2003; 63(18): 5793 - 5798.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
G. P. Margison, A. C. Povey, B. Kaina, and M. F. Santibanez Koref
Variability and regulation of O6-alkylguanine-DNA alkyltransferase
Carcinogenesis, April 1, 2003; 24(4): 625 - 635.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
A.-K. Olsen, H. Bjortuft, R. Wiger, J. Holme, E. Seeberg, M. Bjoras, and G. Brunborg
Highly efficient base excision repair (BER) in human and rat male germ cells
Nucleic Acids Res., April 15, 2001; 29(8): 1781 - 1790.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
L. M. Posnick and L. D. Samson
Influence of S-Adenosylmethionine Pool Size on Spontaneous Mutation, Dam Methylation, and Cell Growth of Escherichia coli
J. Bacteriol., November 1, 1999; 181(21): 6756 - 6762.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
B. P. Engelward, G. Weeda, M. D. Wyatt, J. L. M. Broekhof, J. de Wit, I. Donker, J. M. Allan, B. Gold, J. H. J. Hoeijmakers, and L. D. Samson
Base excision repair deficient mice lacking the Aag alkyladenine DNA glycosylase
PNAS, November 25, 1997; 94(24): 13087 - 13092.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.