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© 1982 Oxford University Press

research-article

Formation of a new population of DNA replication intermediates in X-irradiated human melanoma cells

Ulf Lönn

Department of Histology, Karolinska Institutet and Radiumhemmet, Karolinska Hospital P.O. Box 60400, S-104 01 Stockholm, Sweden

DNA synthesis was investigated in human melanoma cells irradiated with X-rays. The cells were lysed in dilute alkali which results in the release from the parental DNA of replication intermediates; these are then analyzed by agarose gel electrophoresis. In cells irradiated with 10 Gy we can detect the formation of a new heterogeneous population of DNA replication intermediates with a size >10 kb. We can also demonstrate the presence of other DNA replication intermediates which were earlier described in control cells. The results imply that in melanoma cells changes may occur in the replication in order to comply with alterations in the DNA induced by X-rays.


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