Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (120)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Nixon, J. E.
Right arrow Articles by Bailey, G. S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nixon, J. E.
Right arrow Articles by Bailey, G. S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1984 Oxford University Press

research-article

Inhibition of aflatoxin B1 carcinogenesis in rainbow trout by flavone and indole compounds

Joseph E. Nixon, Jerry D. Hendricks, Norman E. Pawlowski, Cliff B. Pereira 1, Russell O. Sinnhuber and George S. Bailey

Department of Food Science and Technology, Oregon State University Corvallis, OR 97331, USA
1Department of Statistics, Oregon State University Corvallis, OR 97331, USA

Several compounds such as flavonoids, selenium, anti-oxidants and retinoids reportedly reduce the induction of cancer in experimental animals, and some have been suggested to function by affecting the mixed-function oxidase (MFO) system. The following compounds: 50 and 500 p.p.m. ß-naphthoflavone (BNF), 1000 p.p.m. flavone, 1000 p.p.m. of a tangeretin-nobilitin mixture, 1000 p.p.m. ß-ionone, 1000 p.p.m. indole-3-carbinol (13C) and 2000 p.p.m. quercetin were examined for protection against aflatoxin B, (AFB1) hepatocardnogenesis, induction of the MFO system and metabolism of AFB1 in rainbow trout. These compounds were fed to fingerting rainbow trout for 8 weeks. At that time the activity of several MFO enzymes and cytochrome P450 content were measured and the trout were exposed for 2 weeks to 20 p.p.b. AFB1 in the same diets. After feeding the test diets without AFB1 for another 6 weeks and basal diet for another 52 weeks, the tumor incidence was determined. The effect of BNF and I3C on in vivo binding of AFB1 to DNA was also measured in separate groups of trout. BNF induced the trout MFO system in a dose-dependent manner, tangeretin-nobilitin was less effective and I3C did not induce. BNF showed significant alterations in the metabolism of AFB1 to aflatoxicol and aflatoxin M1 using cell fractions from pretreated fish. None of the other compounds, including I3C showed such an effect. Despite the apparent lack of in vitro effect of I3C, both BNF and I3C reduced AFB1- DNA binding in vivo. I3C and BNF provided marked protection against AFB induced hepatocardnogenesis, while the other compounds were less effective. The 58 weeks tumor incidences were 4% for 1000 p.p.m. DC, 6% for 500 p.p.m. BNF and 18% for 50 p.p.m. BNF, compared to 38% for the AFB1-positive control. These data demonstrate that gross induction of the MFO system was not necessarily required for alterations in DNA adduct formation in vivo or protection against AFB1 carcinogenesis. Both BNF and I3C provided marked protection but only BNF induced the MFO system.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
G. A. Reed, J. M. Sunega, D. K. Sullivan, J. C. Gray, M. S. Mayo, J. A. Crowell, and A. Hurwitz
Single-Dose Pharmacokinetics and Tolerability of Absorption-Enhanced 3,3'-Diindolylmethane in Healthy Subjects
Cancer Epidemiol. Biomarkers Prev., October 1, 2008; 17(10): 2619 - 2624.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
G. A. Reed, D. W. Arneson, W. C. Putnam, H. J. Smith, J. C. Gray, D. K. Sullivan, M. S. Mayo, J. A. Crowell, and A. Hurwitz
Single-Dose and Multiple-Dose Administration of Indole-3-Carbinol to Women: Pharmacokinetics Based on 3,3'-Diindolylmethane
Cancer Epidemiol. Biomarkers Prev., December 1, 2006; 15(12): 2477 - 2481.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
G. A. Reed, K. S. Peterson, H. J. Smith, J. C. Gray, D. K. Sullivan, M. S. Mayo, J. A. Crowell, and A. Hurwitz
A Phase I Study of Indole-3-Carbinol in Women: Tolerability and Effects
Cancer Epidemiol. Biomarkers Prev., August 1, 2005; 14(8): 1953 - 1960.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol PatholHome page
D. E. Williams, G. S. Bailey, A. Reddy, J. D. Hendricks, A. Oganesian, G. A. Orner, C. B. Pereira, and J. A. Swenberg
The Rainbow Trout (Oncorhynchus mykiss) Tumor Model: Recent Applications in Low-Dose Exposures to Tumor Initiators and Promoters
Toxicol Pathol, January 1, 2003; 31(1_suppl): 58 - 61.
[Abstract] [PDF]

Home page
 CarcinogenesisHome page
G. Stoner, B. Casto, S. Ralston, B. Roebuck, C. Pereira, and G. Bailey
Development of a multi-organ rat model for evaluating chemopreventive agents: efficacy of indole-3-carbinol
Carcinogenesis, February 1, 2002; 23(2): 265 - 272.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
S. A. Larsen-Su and D. E. Williams
Transplacental Exposure to Indole-3-carbinol Induces Sex-Specific Expression of CYP1A1 and CYP1B1 in the Liver of Fischer 344 Neonatal Rats
Toxicol. Sci., December 1, 2001; 64(2): 162 - 168.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.