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© 1985 Oxford University Press

research-article

The role of oral nitrate in the nitrosation of [14C]proline by conventional microflora and germ-free rats

Anthony K. Mallett , Ian R. Rowland , David G. Walters , Sharat D. Gangolli , Richard C. Cottrell  1 and Robert C. Massey 2

The British Industrial Biological Research Association Carshalton, Surrey SM5 4DS
2Ministry of Agriculture, Fisheries and Food Laboratory, Food Science Laboratory Norwich NR2 4SX, UK

The urinary excretion of N-nitroso-L-[U-14C]proline by conventional microflora and germ free rats was used to assess the role of the gut bacteria and oral nitrate in the endogenous formation of N-nitroso compounds. The formation of nitroso-proline was qualitatively similar in conventional and germ-free rats (equivalent to nitrosation of ~0.01–0.05% of the initial dose of [U-14C]proline) suggesting no involvement of the intestinal flora in this reaction. Furthermore, nitrosamino acid production was similar following the administration of nitrate and [U-14C]proline or [U-14C]proline alone, demonstrating no involvement of exogenous nitrate under the conditions of the experiment. Dietary contamination with nitrate/nitrite was negligible. The results are consistent with the suggestion that nitrate/nitrite reserves in the body are important in the formation of nitrosoproline in vivo.


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