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© 1985 Oxford University Press

research-article

The effect of some experimental parameters on the inhibition of metabolic cooperation by phorbol myristate acetate

Thomas G. Hartman and Joseph D. Rosen 1

Department of Food Science, Cook College, New Jersey Agricultural Experiment Station, Rutgers University New Brunswick, NJ 08903, USA

1To whom reprint requests should be sent

The effect of cell density, PMA exposure time, concentration, pre-exposure and binding activity on the recovery of V 79 HGPRT– Chinese hamster cells in the metabolic cooperation assay was determined. A PMA exposure interval of only 1 min resulted in maximum recovery of HGPRT– cells. PMA began to inhibit metabolic cooperation at a dose as low as 0.1 ng/ml final media concentration. The recovery of HGPRT– cells varied according to cell density in the presence or absence of PMA, although the magnitude of this effect was much greater in untreated cells. Pre-exposure of cells to PMA increased the recovery of both post-PMA-treated and non-treated HGPRT– cells in a dose-dependent manner. [3H]PMA was rapidly bound to or taken up by V 79 cells. These results suggest that the inhibitory effect of PMA on metabolic cooperation in V 79 cells involves receptor binding.


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