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© 1985 Oxford University Press

research-article

Interaction between yeast cytochrome P-450 and chemical carcinogens

S.L. Kelly , D.E. Kelly 1, D.J. King 2 and A. Wiseman 2

Wolfson Institute of Biotechnology Sheffield S10 2TN
1Department of Genetics, The University Sheffield S10 2TN
2Biochemistry Division, Department of Biochemistry, University of Surrey Guildford, Surrey GU2 5XH, UK

The binding of various chemical carcinogens to the glucose-inducible form of cytochrome (cyt.) P-450 in Saccharomyces cerevisiae was examined with reference to known metabolism in mammalian systems. All the carcinogens examined gave spectral interactions with the yeast cytochrome. The carcinogens benzo[a]pyrene and cyclophosphamide gave Type I binding, while benzidine and 2-naphthylamine gave Type II binding spectra indicating binding at the haem group. Dimethylnitrosamine gave undetectable binding in yeast microsomal fractions, but a Type I binding spectrum was seen with purified cyt. P-450 preparations. The demonstration that these carcinogens bind to yeast cyt. P-450 is discussed in the light of their genotoxicity.


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