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© 1986 Oxford University Press

other

Reduced number of gap junctions in rat hepatocarcinomas detected by monoclonal antibody

U. Janssen-Timmen , O. Traub , R. Dermietzel 1, H.M. Rabes 2 and K. Willecke 3

Institut fur Zellbiologie Hufelandstrasse 55, Universitat Essen, 43 Essen
1Institut fur Anatomie Hufelandstrasse 55, Universitat Essen, 43 Essen 1
2Pathologisches Institut Thalkirchnerstrasse 36, Universitat Munchen, 8 Munchen 2, FRG

3To whom reprint requests should be sent

A new rat monoclonal antibody was characterized which recognized the 26K protein in gap junctions of mouse, rat and human liver as shown by immunoblot, indirect immuno-fluorescence, and immunogold electron microscopy. This monoclonal antibody was used to investigate the abundance of gap junctions in chemically induced rat hepatocarcinomas. In comparison with livers of control animals we found in hepatocarcinomas an average decrease of 71% in the number of gap junctional immunofluorescent spots. A corresponding decrease of the total amount of the 26 K protein was detected by quantitative immunoblot. Changes in the proliferative state as well as in intercellular adhesion of hepatocarcinoma cells in contrast to normal hepatocytes might have contributed to cause this decrease of gap junctions in tumor tissue. Possibly the partial loss of gap junctions provided a selective advantage for those preneoplastic liver cells which developed into rapidly proliferating tumor cells.


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