© 1987 Oxford University Press
other |
Single GST-P positive liver cells — putative initiated. hepatocytes
1Department of Experimental Pathology, Cancer Institute Kami-Ikebukuro, Toshima-ku, Tokyo 170
2Department of Biochemistry, Hirosaki University School of Medicine Zaifu-cho, Hirosaki 036, Japan
3Present address:Carcinogenesis Research Unit, School of Pathology, University of NSW Kensington 2033, Australia
4To whom reprint requests should be sent
Immunohistochemical investigation of liver tissue 48 h after single doses of the hepatocarcinogens diethylnitrosamine (DEN), dimethylnitrosamine, aflatoxin B1 and methylazoxy-methanol acetate revealed the generation of a population of single glutathione S-transferase placental form (GST-P) positive hepatocytes. Yield was carcinogen dose dependent. Although the mixed function enzyme inducers sodium pheno barbital (PB), methyicholanthrene (Mech), polychlorinated biphenyls (PCB) and isosafrole (IS) did not in themselves induce comparable single cell lesions, their application prior to DEN caused significant alteration in the resultant numbers of GST-P positive hepatocytes observed. While PB and IS were associated with decreased yield, Mech and PCB, in con trast, brought about an increase. The results gained from investigation of the effects of 3-aminobenzamide adminis-tration and partial hepatectomy carried out at different times after carcinogen treatment also pointed to an ‘initiated’ character for the lesions and suggest that GST-P may be a useful marker for analyzing factors relevant to the initiation stage of hepatocarcinogenesis. Thus the interplay between carcino-gen metabolism, DNA adduct formation and repair, toxicity and proliferation may be assessable in terms of numbers of enzyme-altered solitary hepatocytes.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Satoh, D. Yamakawa, H. Sugio, K. Kida, T. Sato, K. Hosoi, and M. Hayakari Bile Duct-bound Growth of Precursor Cells of Preneoplastic Foci Inducible in the Initiation Stage of Rat Chemical Hepatocarcinogenesis by 2-Acetylaminofluorene Jpn. J. Clin. Oncol., September 1, 2008; 38(9): 604 - 610. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Michel, C. Desdouets, M. Slaoui, K. R. Isaacs, R. A. Roberts, and E. Boitier Diethylnitrosamine Initiation Does Not Alter Clofibric Acid Induced Hepatocarcinogenesis in the Rat Toxicol. Sci., September 1, 2007; 99(1): 58 - 69. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Ikeda, K. Omoteyama, K. Yoshida, S. Nishi, and M. Sakai CCAAT Enhancer-binding Protein {alpha} Suppresses the Rat Placental Glutathione S-Transferase Gene in Normal Liver J. Biol. Chem., March 10, 2006; 281(10): 6734 - 6741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Kar, M. Wang, C. S. Wilcox, and B. I. Carr Antitumor and anticarcinogenic actions of Cpd 5: a new class of protein phosphatase inhibitor Carcinogenesis, March 1, 2003; 24(3): 411 - 416. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Satoh and I. Hatayama Anomalous elevation of glutathione S-transferase P-form (GST-P) in the elementary process of epigenetic initiation of chemical hepatocarcinogenesis in rats Carcinogenesis, July 1, 2002; 23(7): 1193 - 1198. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. E. Dean Jr., S. A. Benjamin, L. S. Chubb, J. D. Tessari, and T. J. Keefe Nonadditive Hepatic Tumor Promoting Effects by a Mixture of Two Structurally Different Polychlorinated Biphenyls in Female Rat Livers Toxicol. Sci., March 1, 2002; 66(1): 54 - 61. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Satoh, K. Itoh, M. Yamamoto, M. Tanaka, M. Hayakari, K. Ookawa, T. Yamazaki, T. Sato, S. Tsuchida, and I. Hatayama Nrf2 transactivator-independent GSTP1-1 expression in `GSTP1-1 positive' single cells inducible in female mouse liver by DEN: a preneoplastic character of possible initiated cells Carcinogenesis, March 1, 2002; 23(3): 457 - 462. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Hufnagl, W. Parzefall, B. Marian, M. Kafer, K. Bukowska, R. Schulte-Hermann, and B. Grasl-Kraupp Role of transforming growth factor {alpha} and prostaglandins in preferential growth of preneoplastic rat hepatocytes Carcinogenesis, August 1, 2001; 22(8): 1247 - 1256. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Whysner and C.-X. Wang Hepatocellular Iron Accumulation and Increased Cell Proliferation in Polychlorinated Biphenyl-Exposed Sprague-Dawley Rats and the Development of Hepatocarcinogenesis Toxicol. Sci., July 1, 2001; 62(1): 36 - 45. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Grasl-Kraupp, G. Luebeck, A. Wagner, A. Low-Baselli, M. de Gunst, T. Waldhor, S. Moolgavkar, and R. Schulte-Hermann Quantitative analysis of tumor initiation in rat liver: role of cell replication and cell death (apoptosis) Carcinogenesis, July 1, 2000; 21(7): 1411 - 1421. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Low-Baselli, K. Hufnagl, W. Parzefall, R. Schulte-Hermann, and B. Grasl-Kraupp Initiated rat hepatocytes in primary culture: a novel tool to study alterations in growth control during the first stage of carcinogenesis Carcinogenesis, January 1, 2000; 21(1): 79 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Tessitore Apoptosis and Cell Proliferation Are Involved in the Initiation of Liver Carcinogenesis by a Subnecrogenic Dose of Diethylnitrosamine in Refed Rats J. Nutr., January 1, 2000; 130(1): 104 - 110. [Abstract] [Full Text]
|
|
|
|
|
|
G. A. Wood, D. S.R. Sarma, and M. C. Archer Resistance to the promotion of glutathione S-transferase 7-7-positive liver lesions in Copenhagen rats Carcinogenesis, July 1, 1999; 20(7): 1169 - 1175. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Moore, H. Tsuda, S. Tamano, A. Hagiwara, K. Imaida, T. Shirai, and N. Ito Commentary: Marriage of a Medium-Term Liver Model to Surrogate Markers--A Practical Approach for Risk and Benefit Assessment Toxicol Pathol, March 1, 1999; 27(2): 237 - 242. [Abstract] [PDF]
|
|
|
|
 |
|
 H. C. Pitot The Progression of Neoplasia, Cell Replication, and Electromagnetic Fields International Journal of Toxicology, January 1, 1998; 17(3_suppl): 59 - 108. [PDF]
|
|
|
|
|
|
H. C. Pitot, Y. P Dragan, J. Teeguarden, S. Hsia, and H. Campbell Quantitation of Multistage Carcinogenesis in Rat Liver Toxicol Pathol, January 1, 1996; 24(1): 119 - 128. [Abstract] [PDF]
|
|
|
|
|
|
J. M. Ward, M.-A. Shibata, and D. E. Devor Emerging Issues in Mouse Liver Carcinogenesis Toxicol Pathol, January 1, 1996; 24(1): 129 - 137. [Abstract] [PDF]
|
|
|
|
|
|
D. R. Kemp, D. H. Percy, and M. A. Hayes Lack of Effects of Viral Sialoadenitis and Depletion of Epidermal Growth Factor on Initiation of Hepatic Carcinogenesis in the Rat Toxicol Pathol, February 1, 1991; 19(2): 156 - 163. [Abstract] [PDF]
|
|
|
|
|
|
D. S. Longnecker, N. Chandar, D. G. Sheahan, J. E. Janosky, and B. Lombardi Preneoplastic and Neoplastic Lesions in the Pancreas of Rats Fed Choline-Devoid or Choline-Supplemented Diets Toxicol Pathol, January 1, 1991; 19(1): 59 - 65. [Abstract] [PDF]
|
|