Skip Navigation

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Seifert, J.
Right arrow Articles by Mostecká, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Seifert, J.
Right arrow Articles by Mostecká, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© 1988 Oxford University Press

research-article

Effect of nafenopin and clofibrate on uptake and utilization of labeled thymidine for DNA synthesis in rat liver and kidney

J. Seifert and H. Mostecká

Institute of Pharmacology, Czechoslovak Academy of Sciences Albertov 4, 128 00 Prague 2, Czechoslovakia

The mitogenic effect of nafenopin and clofibrate in the liver is paralleled by a decreased utilization of [14C]thymidine for kidney DNA synthesis. Analogous changes in the liver and kidney DNA biosynthesis after nafenopin administration occur if [14C]orotic acid is added as a precursor of DNA pyrimidines. There is a time correlation between the uptake of labeled thymidine and its utilization for DNA synthesis in the liver during the initial stages of the mitogenic effect of the drug. Later on—after administration of a low dose of nafenopin or of repeated doses of clofibrate—the specific activity of DNA thymine does not differ from the values observed with the control group: the total radioactivity of the thymine components of the acid-soluble extract is even increased. The existence of a correlation between decreased utilization of [14C]thymidine for DNA synthesis and the total radioactivity of the thymine components of the acid-soluble extract in kidney can be observed only during the early stages after nafenopin administration. Subsequently, when the depression of the specific activity of kidney DNA thymine still persists, the specific activity of the thymine components of the acid-soluble extract does not differ from control values. The utilization of [14C]thymidine for DNA synthesis in the kidney is decreased after repeated clofibrate administration only 24 h after the last dose of the drug; these values later increase and even exceed control values. The total radio-activity of the thymine components of the kidney acid-soluble extract is unchanged.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Hum Exp ToxicolHome page
K.N. Woodward
Phthalate Esters, Cystic Kidney Disease in Animals and Possible Effects on Human Health: A Review
Human and Experimental Toxicology, January 1, 1990; 9(6): 397 - 401.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.