© 1989 Oxford University Press
research-article |
Cytotoxic and neoplastic transforming effects of industrial hexavalent chromium pigments in Syrian hamster embryo cells
Institut National de Recherche et de Sècuritè (I.N.R.S.) B.P. 27, 54501 Vandoeuvre Cèdex (France)
1Laboratoire de Rèactivitè de Surface et Structure, U.R.A. 1106-CNRS, 4 place Jussieu, 75252 Paris Gèdex 05 (France)
2Laboratoire de Physico-Chimie Inorganique U.R.A. 419-CNRS, 4 place Jussieu, 75252 Paris Gèdex 05 (France)
Twenty eight moderately water-soluble to insoluble chromium (VI) compounds, such as zinc and lead chromate, industrial and laboratory synthesized pigments, and the analytical reagents strontium, barium and calcium chromate, were physicochemically characterized and studied for cytotoxicity and morphological transformation in cultured Syrian hamster embryo (SHE) cells. In vivo validation of malignancy of transformed SHE cells was performed. A high physico-chemical diversity among the complex chromium pigments was revealed. The solubility of the compounds was greatly increased after incubation in a complete medium and even higher under cell culture conditions. The cytotoxic effects appeared to be due principally to extracellular solubilized chromium because the most solubilized compounds, Zn, Ca and Sr chromates, were equitoxic at about the same Cr concentration treatment and 8-fold more cytotoxic than less soluble compounds such as some Pb chromates and Ba chromate. However, certain physicochemkal properties of lead chromate pigments could also influence their cytotoxic activity. All test compounds were, in a dose-dependent manner, efficient in inducing morphological transformation of SHE cells. Many of the Cr pigments, although physicochemically different, were similarly effective in transformation induction. Nevertheless, compounds among Zn and Pb chromates had various transforming potencies. Ba chromate was the least active in inducing transformation. Certain physicochemkal properties could mediate the transforming activity but no particular relationship could be established between any one of the physicochemical parameters and the transforming potency. Cloned morphologically-transformed colonies of SHE cells were grown in soft agar medium and showed true neoplastic behaviour by tumour formation in syngeneic animals. These results show that various chromate pigments containing either Zn or Pb, of medium to very low aqueous solubility, induced neoplastic transformation of SHE cells.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Z. Elias, O. Poirot, I. Fenoglio, M. Ghiazza, M.-C. Daniere, F. Terzetti, C. Darne, C. Coulais, I. Matekovits, and B. Fubini Surface Reactivity, Cytotoxic, and Morphological Transforming Effects of Diatomaceous Earth Products in Syrian Hamster Embryo Cells Toxicol. Sci., June 1, 2006; 91(2): 510 - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Poznyak, J. M. Puga, E. Kiseleva, and L. Martinez Chromium Toxic Effect Monitoring Using Ozonation Method International Journal of Toxicology, May 1, 2002; 21(3): 211 - 217. [Abstract] [PDF]
|
|
|
|
 |
|
 D. E. Pritchard, S. Ceryak, L. Ha, J. L. Fornsaglio, S. K. Hartman, T. J. O'Brien, and S. R. Patierno Mechanism of Apoptosis and Determination of Cellular Fate in Chromium(VI)-exposed Populations of Telomerase-immortalized Human Fibroblasts Cell Growth Differ., October 1, 2001; 12(10): 487 - 496. [Abstract] [Full Text] [PDF]
|
|