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© 1990 Oxford University Press

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Enhanced preneoplastic liver lesion development under ‘selection pressure’ conditions after administration of deoxycholic or lithocholic acid in the initiation phase in rats

Shunji Kitazawa 1, Ayumi Denda, Masahiro Tsutsumi, Toshifumi Tsujiuchi, Kiyohiko Hasegawa, Kazutoshi Tamura, Hiroshi Maruyama and Yoichi Konishi 2

Department of Oncological Pathology, Cancer Center, Nara Medical College 840 Shijo-cho, Kashihara, Nara 634, Japan

2To whom reprint requests should be sent

The initiating potential of the secondary bile acids, deoxycholic acid (DCA) and lithocholic acid (LCA), for rat hepatocarcino genesis was investigated using the development of hyperplastic nodules and/or glutathione S-transferase placental form (GST-P)-positive liver foci as the end point. Five week old male K344 rats were given either basal diet, or diets containing 0.5% DCA or 0.5% LCA for 3 weeks in conjunction with partial hepatectomy performed midway, followed by a selection regimen consisting of 2 weeks feeding of 0.02% 2-acetylaminofluorene diet and a single gastric intubation of carbon tetrachloride. The animals were then placed on either basal diet or a diet containing 0.05% phenobarbital (PB) for 52 weeks. Significantly higher numbers of hyperplastic liver nodules developed in the DCA-treated rats irrespective of PB promotion as compared with the respective control groups. No such increase was evident in the LCA-treated rats. In contrast, both DCA and LCA treatments enhanced the development of GST-P-posltlve liver foci with or without subsequent PB promotion. Only one hepatocellular carcinoma was diagnosed in a control group animal. The present data indicate that a short period of feeding of DCA and LCA in the initiation stage in conjunction with partial hepatectomy results in enhanced development of preneoplastic liver lesions under selection pressure conditions with or without subsequent PB promotion. They thus confirm and extend our previous finding of enhanced {gamma}-glutamyttranspeptidase positive liver foci development in a short-term assay of DCA and LCA, and suggest that these secondary bile acids either possess possible initiating activity or some other priming effect for rat hepatocarcinogenesis.


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