Carcinogenesis, Vol 18, 627-632, Copyright © 1997 by Oxford University Press
JL Fang and CE Vaca
The effect of alcohol drinking on the formation of DNA adducts of
acetaldehyde, the primary oxidative metabolite of ethanol, was investigated
in humans. DNA was isolated from granulocytes and lymphocytes from 24
alcoholic patients and 12 control subjects. DNA adduct levels were measured
by 32P-postlabelling using reversed-phase HPLC with on-line detection of
radioactivity. A large interindividual variation in adduct levels was
observed. The average adduct levels in granulocyte and lymphocyte DNA from
alcoholic patients were 3.4 +/- 3.8 and 2.1 +/- 0.8 adducts/10(7)
nucleotides (n = 24), respectively. These levels were 13- and 7-fold higher
than the corresponding levels in control subjects (P<0.001). The average
adduct level in granulocyte DNA from alcoholic patients was 60% higher than
in lymphocyte DNA (P<0.01). Our results, in conjunction with the
genotoxicity of acetaldehyde, thus suggest the formation of DNA adducts of
acetaldehyde as a plausible mechanism explaining the involvement of alcohol
drinking in carcinogenesis.
ARTICLES
Detection of DNA adducts of acetaldehyde in peripheral white blood cells of alcohol abusers
Department of Biosciences at NOVUM, Karolinska Institute, Huddinge, Sweden.
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