Carcinogenesis, Vol. 20, No. 11, 2109-2115,
November 1999
© 1999 Oxford University Press
Molecular Epidemiology and Cancer Prevention |
Dietary prevention of azoxymethane-induced colon carcinogenesis with rice-germ in F344 rats
First Department of Pathology, Gifu University School of Medicine, 40 Tsukasa-machi, Gifu 500-8705,
1 First Department of Pathology, Kanazawa Medical University, 1-1 Daigaku, Uchinada, Ishikawa 920-0293,
2 Oryza Oil and Fat Chemical Co. Ltd, Ichinomiya 493-8001 and
3 Department of Biochemistry, Gifu Pharmaceutical University, 5-6-1 Mitahorahigashi, Gifu 502-0003, Japan
The modifying effect of dietary administration of defatted rice-germ and 
-aminobutyric acid (GABA)-enriched defatted rice-germ on azoxymethane (AOM)-induced colon carcinogenesis was investigated in two experiments with male F344 rats. In the first experiment (the pilot study), the effects of the defatted rice-germ, the GABA-enriched defatted rice-germ and rice-germ on AOM-induced (15 mg/kg body wt once a week for 3 weeks) formation of aberrant crypt foci (ACF) were examined. The latter two preparations (2.5% in the diet) significantly inhibited ACF formation (P < 0.005). In the second experiment, a long-term study of the effects of rice-germ was done. One group was treated with AOM alone, four groups received the carcinogen and were fed the diets containing 2.5% rice-germ or 2.5% GABA-enriched defatted rice-germ for 5 (initiation phase) or 30 weeks (post-initiation phase), two groups were treated with rice-germ or GABA-enriched defatted rice-germ alone and one group was kept on the basal diet. At the termination of the study, dietary exposure to rice-germ during the initiation phase significantly reduced the incidence of colonic adenocarcinoma (71 versus 29%, P < 0.01). GABA-enriched defatted rice-germ or rice-germ during the post-initiation phase also decreased the frequency of colonic adenocarcinoma (71 versus 20%, GABA-enriched defatted rice-germ feeding, P < 0.01; 27%, rice-germ feeding, P < 0.01). These data suggest that constituents of rice-germ are possible dietary preventatives for human colon cancers.
Abbreviations: ACF, aberrant crypt foci; AOM, azoxymethane; GABA, -aminobutyric acid; PCNA, proliferating cell nuclear antigen.
4 To whom correspondence should be addressed. Email: hidmori{at}cc.gifu-u.ac.jp
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