Carcinogenesis, Vol. 20, No. 11, 2117-2124,
November 1999
© 1999 Oxford University Press
Molecular Epidemiology and Cancer Prevention |
Prevention of UVB-induced immunosuppression in mice by the green tea polyphenol (–)-epigallocatechin-3-gallate may be associated with alterations in IL-10 and IL-12 production
Department of Dermatology, Case Western Reserve University, 11100 Euclid Avenue, Cleveland and University Hospitals of Cleveland and
1 VA Hospital, Cleveland, OH 44106, USA
UV exposure of the skin, particularly UVB (290–320 nm), causes adverse biological effects, including alterations in cutaneous immune cells, photoaging and photocarcinogenesis. Several studies have shown that polyphenolic compounds isolated from green tea afford protection against UVB-induced inflammatory responses and photocarcinogenesis in murine models. In this study we show that topical application of (–)-epigallocatechin-3-gallate (EGCG) (3 mg/mouse), a major polyphenolic component of green tea, before a single low dose UVB exposure (72 mJ/cm2) to C3H/HeN mice prevented UVB-induced inhibition of the contact hypersensitivity response and tolerance induction to the contact sensitizer 2,4-dinitrofluorobenzene. Topical application of EGCG before UVB exposure reduced the number of CD11b+ monocytes/macrophages and neutrophils infiltrating into skin inflammatory lesions, which are considered to be responsible for creating the UV-induced immunosuppressive state. In addition, application of EGCG before UVB exposure decreased UVB-induced production of the immunomodulatory cytokine interleukin (IL)-10 in skin as well as in draining lymph nodes (DLN), whereas production of IL-12, which is considered to be a mediator and adjuvant for induction of contact sensitivity, was found to be markedly increased in DLN when compared with UVB alone-exposed mice. Taken together, our data demonstrate that EGCG protects against UVB-induced immunosuppression and tolerance induction by: (i) blocking UVB-induced infiltration of CD11b+ cells into the skin; (ii) reducing IL-10 production in skin as well as in DLN; (iii) markedly increasing IL-12 production in DLN. Protection against UVB-induced immunosuppression by EGCG may be associated with protection against UVB-induced photocarcinogenesis.
Abbreviations: APC, antigen-presenting cell; CHS, contact hypersensitivity; DAB, diaminobenzidine; DLN, draining lymph node; DNFB, 2,4-dinitrofluorobenzene; EGCG, (–)-epigallocatechin-3-gallate; FBS, fetal bovine serum; IL, interleukin.
2 To whom correspondence should be addressed Email: sxk32{at}po.cwru.edu
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  E. Zattra, C. Coleman, S. Arad, E. Helms, D. Levine, E. Bord, A. Guillaume, M. El-Hajahmad, E. Zwart, H. van Steeg, et al. Polypodium leucotomos Extract Decreases UV-Induced Cox-2 Expression and Inflammation, Enhances DNA Repair, and Decreases Mutagenesis in Hairless Mice Am. J. Pathol., November 1, 2009; 175(5): 1952 - 1961. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Meeran, M. Vaid, T. Punathil, and S. K. Katiyar Dietary grape seed proanthocyanidins inhibit 12-O-tetradecanoyl phorbol-13-acetate-caused skin tumor promotion in 7,12-dimethylbenz[a]anthracene-initiated mouse skin, which is associated with the inhibition of inflammatory responses Carcinogenesis, March 1, 2009; 30(3): 520 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Meeran, S. Katiyar, C. A. Elmets, and S. K. Katiyar Interleukin-12 Deficiency Is Permissive for Angiogenesis in UV Radiation-Induced Skin Tumors Cancer Res., April 15, 2007; 67(8): 3785 - 3793. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Sharma, S. M. Meeran, and S. K. Katiyar Dietary grape seed proanthocyanidins inhibit UVB-induced oxidative stress and activation of mitogen-activated protein kinases and nuclear factor-{kappa}B signaling in in vivo SKH-1 hairless mice Mol. Cancer Ther., March 1, 2007; 6(3): 995 - 1005. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Meeran, S. Katiyar, C. A. Elmets, and S. K. Katiyar Silymarin inhibits UV radiation-induced immunosuppression through augmentation of interleukin-12 in mice. Mol. Cancer Ther., July 1, 2006; 5(7): 1660 - 1668. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Meeran, S. K. Mantena, C. A. Elmets, and S. K. Katiyar (-)-Epigallocatechin-3-Gallate Prevents Photocarcinogenesis in Mice through Interleukin-12-Dependent DNA Repair. Cancer Res., May 15, 2006; 66(10): 5512 - 5520. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. M. Meeran, S. K. Mantena, S. Meleth, C. A. Elmets, and S. K. Katiyar Interleukin-12-deficient mice are at greater risk of UV radiation-induced skin tumors and malignant transformation of papillomas to carcinomas. Mol. Cancer Ther., April 1, 2006; 5(4): 825 - 832. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Meeran, S. K. Mantena, and S. K. Katiyar Prevention of Ultraviolet Radiation-Induced Immunosuppression by (-)-Epigallocatechin-3-Gallate in Mice Is Mediated through Interleukin 12-Dependent DNA Repair. Clin. Cancer Res., April 1, 2006; 12(7): 2272 - 2280. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.-M. Huang, C.-C. Wang, M. Kawai, S. Barnes, and C. A. Elmets Surfactant Sodium Lauryl Sulfate Enhances Skin Vaccination: Molecular Characterization via a Novel Technique using Ultrafiltration Capillaries and Mass Spectrometric Proteomics Mol. Cell. Proteomics, March 1, 2006; 5(3): 523 - 532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. D. Sharma and S. K. Katiyar Dietary grape-seed proanthocyanidin inhibition of ultraviolet B-induced immune suppression is associated with induction of IL-12 Carcinogenesis, January 1, 2006; 27(1): 95 - 102. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. S. Haddad, G. A. Azar, S. Groom, and M. Boivin Natural Health Products, Modulation of Immune Function and Prevention of Chronic Diseases Evid. Based Complement. Altern. Med., December 1, 2005; 2(4): 513 - 520. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Mantena, S. M. Meeran, C. A. Elmets, and S. K. Katiyar Orally Administered Green Tea Polyphenols Prevent Ultraviolet Radiation-Induced Skin Cancer in Mice through Activation of Cytotoxic T Cells and Inhibition of Angiogenesis in Tumors J. Nutr., December 1, 2005; 135(12): 2871 - 2877. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Rogers, I. Perkins, A. van Olphen, N. Burdash, T. W. Klein, and H. Friedman Epigallocatechin Gallate Modulates Cytokine Production by Bone Marrow-Derived Dendritic Cells Stimulated with Lipopolysaccharide or Muramyldipeptide, or Infected with Legionella pneumophila Experimental Biology and Medicine, October 1, 2005; 230(9): 645 - 651. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. K. Vayalil, C. A. Elmets, and S. K. Katiyar Treatment of green tea polyphenols in hydrophilic cream prevents UVB-induced oxidation of lipids and proteins, depletion of antioxidant enzymes and phosphorylation of MAPK proteins in SKH-1 hairless mouse skin Carcinogenesis, May 1, 2003; 24(5): 927 - 936. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Dona, I. Dell'Aica, F. Calabrese, R. Benelli, M. Morini, A. Albini, and S. Garbisa Neutrophil Restraint by Green Tea: Inhibition of Inflammation, Associated Angiogenesis, and Pulmonary Fibrosis J. Immunol., April 15, 2003; 170(8): 4335 - 4341. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Matsunaga, T. W. Klein, H. Friedman, and Y. Yamamoto Epigallocatechin Gallate, a Potential Immunomodulatory Agent of Tea Components, Diminishes Cigarette Smoke Condensate-Induced Suppression of Anti-Legionella pneumophila Activity and Cytokine Responses of Alveolar Macrophages Clin. Vaccine Immunol., July 1, 2002; 9(4): 864 - 871. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. TOSETTI, N. FERRARI, S. DE FLORA, and A. ALBINI Angioprevention': angiogenesis is a common and key target for cancer chemopreventive agents FASEB J, January 1, 2002; 16(1): 2 - 14. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Matsunaga, T. W. Klein, H. Friedman, and Y. Yamamoto Legionella pneumophila Replication in Macrophages Inhibited by Selective Immunomodulatory Effects on Cytokine Formation by Epigallocatechin Gallate, a Major Form of Tea Catechins Infect. Immun., June 1, 2001; 69(6): 3947 - 3953. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. K. Katiyar and H. Mukhtar Green tea polyphenol (-)-epigallocatechin-3-gallate treatment to mouse skin prevents UVB-induced infiltration of leukocytes, depletion of antigen-presenting cells, and oxidative stress J. Leukoc. Biol., May 1, 2001; 69(5): 719 - 726. [Abstract] [Full Text]
|
|
|
|
|
|
S. K. Katiyar, F. Afaq, A. Perez, and H. Mukhtar Green tea polyphenol (-)-epigallocatechin-3-gallate treatment of human skin inhibits ultraviolet radiation-induced oxidative stress Carcinogenesis, February 1, 2001; 22(2): 287 - 294. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Budiyanto, N. U. Ahmed, A. Wu, T. Bito, O. Nikaido, T. Osawa, M. Ueda, and M. Ichihashi Protective effect of topically applied olive oil against photocarcinogenesis following UVB exposure of mice Carcinogenesis, November 1, 2000; 21(11): 2085 - 2090. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Sluyter and G. M. Halliday Enhanced tumor growth in UV-irradiated skin is associated with an influx of inflammatory cells into the epidermis Carcinogenesis, October 1, 2000; 21(10): 1801 - 1807. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. K. Katiyar, A. Perez, and H. Mukhtar Green Tea Polyphenol Treatment to Human Skin Prevents Formation of Ultraviolet Light B-induced Pyrimidine Dimers in DNA Clin. Cancer Res., October 1, 2000; 6(10): 3864 - 3869. [Abstract] [Full Text]
|
|
|
|
 |
|
 S. K. Katiyar, N. Ahmad, and H. Mukhtar Green Tea and Skin Arch Dermatol, August 1, 2000; 136(8): 989 - 994. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Nam, D. M. Smith, and Q. P. Dou Ester Bond-containing Tea Polyphenols Potently Inhibit Proteasome Activity in Vitro and in Vivo J. Biol. Chem., April 13, 2001; 276(16): 13322 - 13330. [Abstract] [Full Text] [PDF]
|
|