Carcinogenesis, Vol. 20, No. 3, 431-435,
March 1999
© 1999 Oxford University Press
HPLC/fluorescence determination of anti-BPDE–DNA adducts in mononuclear white blood cells from PAH-exposed humans
1 Institute of Occupational Health, University of Padova, Via Giustiniani 2, 35128 Padova,
2 CNR, Area di Ricerca, Corso Stati Uniti 4, Padova,
3 Institute of Occupational Health, University of Verona, Verona and
4 Occupational Health Service, Provincia di Bolzano, Boleano, Italy
The aim of this study was to compare (±)-r-7,t-8-dihydroxy-t-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene (anti-BPDE)–DNA adduct levels in groups of humans subjected to various levels of polycyclic aromatic hydrocarbon (PAH) (benzo[a]pyrene) exposure. An HPLC/fluorescence method was applied to detect specifically anti-BPDE–DNA adducts in mononuclear white blood cells [lymphocyte plus monocyte fraction (LMF)] from humans exposed to PAHs. A total of 130 subjects comprised the sample population: 26 psoriatic patients (3 days after clinical coal tar treatment of the skin), 15 coke oven workers, 19 chimney sweeps, 36 aluminium anode plant workers and 34 non-occupationally PAH-exposed subjects (controls). PAH exposure was assessed in each group by means of the urinary excretion of 1-pyrenol (mean group levels: 1.2, 0.7, 0.3, 65.0 and 0.1 µmol/mol creatinine in coke oven workers, chimney sweeps, aluminium plant anode workers, psoriatic patients and non-occupationally PAH-exposed subjects, respectively). HPLC/fluorescence analysis of BPDE–DNA adducts showed that the percentage of subjects with adduct levels exceeding the 95 percentile control subject value (8.9 adducts/108 nucleotides) was significantly high in coke oven workers (46.7%) and chimney sweeps (21.0%) (
2 test, P < 0.01 and P < 0.05, respectively) but not in aluminium plant workers (11.1%) and psoriatic patients (0%). The increase in BPDE–DNA adduct levels in LMF (Ln values) was significantly related to chronic inhalatory and high PAH exposure (linear multiple regression analysis, F = 6.37, P < 0.01; t = 4.2, P < 0.001). Skin acute (or short-term) and high PAH exposure, charcoal-grilled meat consumption and smoking habit did not seem to influence BPDE–DNA adduct formation in LMF.
Abbreviations: anti-BPDE, (±)-r-7,t-8-dihydroxy-t-9,10-oxy-7,8,9,10-tetrahydrobenzo[a]pyrene; B[a]P, benzo[a]pyrene; B[a]P-tetrol I-1, r-7,c-10,t-8,t-9-tetrahydroxy-7,8,9,10-tetrahydro-benzo[a]pyrene; CT, coal tar; CYP, cytochrome P450; GST, glutathione S-transferase; LMF, lymphocyte plus monocyte fraction; PAH, polycyclic aromatic hydrocarbon; TLC, thin-layer chromatography; WBC, white blood cell.
5 To whom correspondence should be addressed Email: clonfero{at}uxl.unipd.it
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Final Safety Assessment of Coal Tar as Used in Cosmetics International Journal of Toxicology, March 1, 2008; 27(2_suppl): 1 - 24. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Gyorffy, L. Anna, K. Kovacs, P. Rudnai, and B. Schoket Correlation between biomarkers of human exposure to genotoxins with focus on carcinogen-DNA adducts Mutagenesis, January 1, 2008; 23(1): 1 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-J. An, J.-K. Chen, H.-j. Chen, W. Chang, Y.-G. Jiang, Q.-Y. Wei, and X.-M. Chen Characterization of 67 kD Laminin Receptor, a Protein Whose Gene Is Overexpressed on Treatment of Cells with Anti-Benzo[a]pyrene-7,8-Diol-9,10-Epoxide Toxicol. Sci., April 1, 2006; 90(2): 326 - 330. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Culea, O. Cozar, and E. Culea PAHs in Cigarette Smoke by Gas Chromatography-Mass Spectrometry Indoor and Built Environment, June 1, 2005; 14(3-4): 283 - 292. [Abstract] [PDF]
|
|
|
|
 |
|
 S. Pavanello, A. Pulliero, E. Siwinska, D. Mielzynska, and E. Clonfero Reduced nucleotide excision repair and GSTM1-null genotypes influence anti-B[a]PDE-DNA adduct levels in mononuclear white blood cells of highly PAH-exposed coke oven workers Carcinogenesis, January 1, 2005; 26(1): 169 - 175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. H. Phillips Smoking-related DNA and protein adducts in human tissues Carcinogenesis, December 1, 2002; 23(12): 1979 - 2004. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Schwerdtle, A. Seidel, and A. Hartwig Effect of soluble and particulate nickel compounds on the formation and repair of stable benzo[a]pyrene DNA adducts in human lung cells Carcinogenesis, January 1, 2002; 23(1): 47 - 53. [Abstract] [Full Text] [PDF]
|
|