Carcinogenesis, Vol. 20, No. 4, 733-736,
April 1999
© 1999 Oxford University Press
Short Communications |
Suppression of extracellular signals and cell proliferation by the black tea polyphenol, theaflavin-3,3'-digallate
Institutes of
1 Biochemistry and
2 Toxicology, College of Medicine, National Taiwan University, No. 1, Section 1, Jen-Ai Road, Taipei, Taiwan, ROC and
3 Department of Food Science, Cook College, Rutgers, State University of New Jersey, NJ, USA
Previous studies in our laboratory have shown that the major green tea polyphenol, (–)-epigallocatechin-3-gallate (EGCG), suppressed autophosphorylation of epidermal growth factor (EGF) receptor induced by EGF in human A431 epidermoid carcinoma cells. In this study, we examined the inhibitory effects of black tea polyphenols, including theaflavin (TF-1), a mixture (TF-2) of theaflavin-3-gallate (TF-2a) and theaflavin-3'-gallate (TF-2b), theaflavin-3,3'-digallate (TF-3) and the thearubigin fraction on the autophosphorylation of the EGF and PDGF receptors in A431 cells and mouse NIH3T3 fibroblast cells, respectively. First, we examined the effects of these polyphenols on the proliferation of A431 and NIH3T3 cells. Both EGCG and TF-3 strongly inhibited the proliferation of A431 and NIH3T3 cells more than the other theaflavins did. In cultured cells with pre-treatment of tea polyphenol, TF-3 was stronger than EGCG on the reduction of EGF receptor and PDGF receptor autophosphorylation induced by EGF and PDGF, respectively. Other theaflavins slightly reduced the autophosphorylation of the EGF and PDGF receptors; furthermore, TF-3 could reduce autophosphorylation of the EGF receptor (or PDGF receptor) even with co-treatment with EGF (or PDGF) and TF-3, but EGCG was inactive under these conditions. In addition, TF-3 was stronger than EGCG in blocking EGF binding to its receptor. These results suggest that not only the green tea polyphenol, EGCG, but also the black tea polyphenol, TF-3, have an antiproliferative activity on tumor cells, and the molecular mechanisms of antiproliferation may block the growth factor binding to its receptor and thus suppress mitogenic signal transduction.
Abbreviations: DMEM, Dulbecco's modified essential medium; EGCG, (–)-epigallocatechin-3-gallate; EGF, epidermal growth factor; FBS, fetal bovine serum; PDGF, platelet-derived growth factor; SDS, sodium dodecyl sulfate; TF-1, theaflavin; TF-2, mixture of theaflavin-3-gallate and theaflavin-3'-gallate; TF-2a, theaflavin-3-gallate; TF-2b, theaflavin-3'-gallate; TF-3, theaflavin-3,3'-digallate; TR, thearubigin.
3 To whom correspondence should be addressed Email:jenkun{at}ntumc1.mc.ntu.edu.tw
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  N. Kumar, L. Titus-Ernstoff, P. A. Newcomb, A. Trentham-Dietz, G. Anic, and K. M. Egan Tea Consumption and Risk of Breast Cancer Cancer Epidemiol. Biomarkers Prev., January 1, 2009; 18(1): 341 - 345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Halder, U. Bhattacharya, S. Mukhopadhyay, and A. K. Giri Molecular mechanism of black tea polyphenols induced apoptosis in human skin cancer cells: involvement of Bax translocation and mitochondria mediated death cascade Carcinogenesis, January 1, 2008; 29(1): 129 - 138. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Adachi, T. Nagao, H. I. Ingolfsson, F. R. Maxfield, O. S. Andersen, L. Kopelovich, and I. B. Weinstein The Inhibitory Effect of (-)-Epigallocatechin Gallate on Activation of the Epidermal Growth Factor Receptor Is Associated with Altered Lipid Order in HT29 Colon Cancer Cells Cancer Res., July 1, 2007; 67(13): 6493 - 6501. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Stangl, H. Dreger, K. Stangl, and M. Lorenz Molecular targets of tea polyphenols in the cardiovascular system Cardiovasc Res, January 15, 2007; 73(2): 348 - 358. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Hou, S. Sang, H. You, M.-J. Lee, J. Hong, K.-V. Chin, and C. S. Yang Mechanism of Action of (-)-Epigallocatechin-3-Gallate: Auto-oxidation-Dependent Inactivation of Epidermal Growth Factor Receptor and Direct Effects on Growth Inhibition in Human Esophageal Cancer KYSE 150 Cells Cancer Res., September 1, 2005; 65(17): 8049 - 8056. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Ermakova, B. Y. Choi, H. S. Choi, B. S. Kang, A. M. Bode, and Z. Dong The Intermediate Filament Protein Vimentin Is a New Target for Epigallocatechin Gallate J. Biol. Chem., April 29, 2005; 280(17): 16882 - 16890. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Vittal, Z. E. Selvanayagam, Y. Sun, J. Hong, F. Liu, K.-V. Chin, and C. S. Yang Gene expression changes induced by green tea polyphenol (-)-epigallocatechin-3-gallate in human bronchial epithelial 21BES cells analyzed by DNA microarray Mol. Cancer Ther., September 1, 2004; 3(9): 1091 - 1099. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-H. Lee, C.-T. Ho, and J.-K. Lin Theaflavin-3,3'-digallate and penta-O-galloyl-{beta}-D-glucose inhibit rat liver microsomal 5{alpha}-reductase activity and the expression of androgen receptor in LNCaP prostate cancer cells Carcinogenesis, July 1, 2004; 25(7): 1109 - 1118. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. S. Wheeler, J. D. Catravas, K. Odoms, A. Denenberg, V. Malhotra, and H. R. Wong Epigallocatechin-3-gallate, a Green Tea-Derived Polyphenol, Inhibits IL-1{beta}-Dependent Proinflammatory Signal Transduction in Cultured Respiratory Epithelial Cells J. Nutr., May 1, 2004; 134(5): 1039 - 1044. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Leone, D. Zhai, S. Sareth, S. Kitada, J. C. Reed, and M. Pellecchia Cancer Prevention by Tea Polyphenols Is Linked to Their Direct Inhibition of Antiapoptotic Bcl-2-Family Proteins Cancer Res., December 1, 2003; 63(23): 8118 - 8121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. A. Orner, W.-M. Dashwood, C. A. Blum, G. D. Diaz, Q. Li, and R. H. Dashwood Suppression of tumorigenesis in the Apcmin mouse: down-regulation of {beta}-catenin signaling by a combination of tea plus sulindac Carcinogenesis, February 1, 2003; 24(2): 263 - 267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Klein and S. M. Fischer Black tea polyphenols inhibit IGF-I-induced signaling through Akt in normal prostate epithelial cells and Du145 prostate carcinoma cells Carcinogenesis, January 1, 2002; 23(1): 217 - 221. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Masuda, M. Suzui, and I. B. Weinstein Effects of Epigallocatechin-3-gallate on Growth, Epidermal Growth Factor Receptor Signaling Pathways, Gene Expression, and Chemosensitivity in Human Head and Neck Squamous Cell Carcinoma Cell Lines Clin. Cancer Res., December 1, 2001; 7(12): 4220 - 4229. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lu, C.-T. Ho, G. Ghai, and K. Y. Chen Differential Effects of Theaflavin Monogallates on Cell Growth, Apoptosis, and Cox-2 Gene Expression in Cancerous versus Normal Cells Cancer Res., November 1, 2000; 60(22): 6465 - 6471. [Abstract] [Full Text]
|
|