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Carcinogenesis, Vol. 20, No. 8, 1529-1534, August 1999
© 1999 Oxford University Press

Carcinogenesis

Alterations of p53 in tumorigenic human bronchial epithelial cells correlate with metastatic potential

Chang Q. Piao, James C. Willey1 and Tom K. Hei2

Center for Radiological Research, College of Physicians and Surgeons of Columbia University, 630 West 168th Street, New York, NY 10032 and
1 Department of Medicine, Medical College of Ohio, Toledo, OH 43699, USA

The cellular and molecular mechanisms of radiation-induced lung cancer are not known. In the present study, alterations of p53 in tumorigenic human papillomavirus-immortalized human bronchial epithelial (BEP2D) cells induced by a single low dose of either {alpha}-particles or 1 GeV/nucleon 56Fe were analyzed by PCR–single-stranded conformation polymorphism (SSCP) coupled with sequencing analysis and immunoprecipitation assay. A total of nine primary and four secondary tumor cell lines, three of which were metastatic, together with the parental BEP2D and primary human bronchial epithelial (NHBE) cells were studied. The immunoprecipitation assay showed overexpression of mutant p53 proteins in all the tumor lines but not in NHBE and BEP2D cells. PCR–SSCP and sequencing analysis found band shifts and gene mutations in all four of the secondary tumors. A G->T transversion in codon 139 in exon 5 that replaced Lys with Asn was detected in two tumor lines. One mutation each, involving a G->T transversion in codon 215 in exon 6 (Ser->lle) and a G->A transition in codon 373 in exon 8 (Arg->His), was identified in the remaining two secondary tumors. These results suggest that p53 alterations correlate with tumorigenesis in the BEP2D cell model and that mutations in the p53 gene may be indicative of metastatic potential.

Abbreviations: Rb, retinoblastoma; SSCP, single-stranded conformation polymorphism

2 To whom correspondence should be addressedEmail: tkh1{at}Columbia.edu


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