Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (28)
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Takihara, Y.
Right arrow Articles by Hara, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Takihara, Y.
Right arrow Articles by Hara, J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Carcinogenesis, Vol. 21, No. 11, 2073-2077, November 2000
© 2000 Oxford University Press

Carcinogenesis

Role of the ß isoform of 14-3-3 proteins in cellular proliferation and oncogenic transformation

Yoshihiro Takihara2, Yoshiko Matsuda1 and Junichi Hara1

Department of Medical Genetics and Molecular Cell Biology, Research Institute for Microbial Diseases, Osaka University and
1 Department of Developmental Medicine, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan

The 14-3-3 proteins are associated with proto-oncogene and oncogene products. Here, we generated NIH 3T3 cells overexpressing the ß isoform of the 14-3-3 proteins (14-3-3 ß) to examine the function of this isoform in cellular proliferation and oncogenic transformation. Overexpression of 14-3-3 ß in NIH 3T3 cells stimulated cell growth and supported anchorage-independent growth in soft agar medium and tumor formation in nude mice. To elucidate the molecular mechanisms of 14-3-3 ß-mediated NIH 3T3 transformation, we examined the activity of mitogen-activated protein kinase (MAPK) after serum stimulation. Overexpression of 14-3-3 ß augmented MAPK activity after serum stimulation, and MAPK activity correlated well with the amount of 14-3-3 ß expression. The colony-forming ability of NIH 3T3 cells overexpressing 14-3-3 ß in soft agar medium was efficiently abolished by exogenous expression of a dominant-negative mutant of MEK1 and 14-3-3 ß physically interacted with Raf-1 in these cells. These findings indicate that 14-3-3 ß has oncogenic potential, mainly through enhancement of Raf-1 activation and resultant augmentation of signaling in the MAPK cascade.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. A. Maxwell, Z. Li, D. Jaya, S. Ballard, J. Ferrell, and H. Fu
14-3-3{zeta} Mediates Resistance of Diffuse Large B Cell Lymphoma to an Anthracycline-based Chemotherapeutic Regimen
J. Biol. Chem., August 14, 2009; 284(33): 22379 - 22389.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
B. S. Ajjappala, Y.-S. Kim, M.-S. Kim, M.-Y. Lee, K.-Y. Lee, H.-Y. Ki, D.-H. Cha, and K.-H. Baek
14-3-3{gamma} Is Stimulated by IL-3 and Promotes Cell Proliferation
J. Immunol., January 15, 2009; 182(2): 1050 - 1060.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. Li, J. Zhao, Y. Du, H. R. Park, S.-Y. Sun, L. Bernal-Mizrachi, A. Aitken, F. R. Khuri, and H. Fu
Down-regulation of 14-3-3{zeta} suppresses anchorage-independent growth of lung cancer cells through anoikis activation
PNAS, January 8, 2008; 105(1): 162 - 167.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
A. Sugiyama, Y. Miyagi, Y. Komiya, N. Kurabe, C. Kitanaka, N. Kato, Y. Nagashima, Y. Kuchino, and F. Tashiro
Forced expression of antisense 14-3-3{beta} RNA suppresses tumor cell growth in vitro and in vivo
Carcinogenesis, September 1, 2003; 24(9): 1549 - 1559.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
D. Martin, M. Brown-Luedi, and R. Chiquet-Ehrismann
Tenascin-C signaling through induction of 14-3-3 tau
J. Cell Biol., January 21, 2003; 160(2): 171 - 175.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. ProteomicsHome page
S. Gharbi, P. Gaffney, A. Yang, M. J. Zvelebil, R. Cramer, M. D. Waterfield, and J. F. Timms
Evaluation of Two-dimensional Differential Gel Electrophoresis for Proteomic Expression Analysis of a Model Breast Cancer Cell System
Mol. Cell. Proteomics, February 1, 2002; 1(2): 91 - 98.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.