Carcinogenesis, Vol. 21, No. 11, 2123-2128,
November 2000
© 2000 Oxford University Press
Short Communication |
Ethanol and acetaldehyde enhance benzo[a]pyrene–DNA adduct formation in human mammary epithelial cells
Department of Food Science and Human Nutrition, University of Illinois, Urbana, IL 61801, USA
Abbreviations: B[a]P, benzo[a]pyrene; dAdo, deoxyadenosine; dGuo, deoxyguanosine; DMBA, 7,12-dimethylbenz[a]anthracene; EROD, ethoxyresorufin O-deethylase; GST, glutathione S-transferase; QR, NAD(P)H-quinone oxidoreductase; PAH, polycyclic aromatic hydrocarbon.
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Introduction |
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A positive association between alcohol intake and breast cancer risk has been found in many epidemiological studies, yet the biological basis for this association is incompletely understood. The present experiments were designed to determine the chronic effect of physiologically relevant concentrations of ethanol (5–25 mM) and acetaldehyde (0.5–5.0 µM) on benzo[a]pyrene (B[a]P)–DNA adduct formation in the non-neoplastic human mammary epithelial cell line, MCF-10F. We tentatively identified the major adduct formed as the anti-dihydrodiol epoxide of B[a]P bound to deoxyguanosine (dGuo). For these studies, cells were treated with ethanol or acetaldehyde for 6 days before exposure to B[a]P at 0.02 or 0.08 µg/ml. Cells incubated with B[a]P at 0.08 µg/ml for 12 h after 6 days' exposure to 15 and 25 mM ethanol exhibited 1.3- and 2.2-fold increases in B[a]P–DNA adduct formation, respectively. MCF-10F cells treated with B[
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Acknowledgments |
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References |
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